PGF1 Mouse

Placental Growth Factor-1 Mouse Recombinant
Cat. No.
BT28053
Source
Sf9, Baculovirus cells.
Synonyms
Placenta growth factor, PlGF, PGF1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PGF1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 138 amino acids (27-158a.a.) and having a molecular mass of 15.9kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
PGF1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Placental Growth Factor (PGF) is highly concentrated in trophoblastic giant cells, which are connected to the parietal yolk sac during the initial stages of embryo development. PGF secretion from these cells acts as a trigger and coordinator for the formation of blood vessels in the deciduum and placenta during early embryogenesis.
Description
Recombinant Mouse PGF1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 138 amino acids (27-158a.a.), resulting in a molecular mass of 15.9kDa. Note that on SDS-PAGE, the apparent molecular size will be approximately 18-28kDa. PGF1 is expressed with a C-terminal 6-amino acid His tag and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The PGF1 protein solution (1mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) containing 0.1mM PMSF and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Placenta growth factor, PlGF, PGF1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
AGNNSTEVEV VPFNEVWGRS YCRPMEKLVY ILDEYPDEVS HIFSPSCVLL SRCSGCCGDE GLHCVPIKTA NITMQILKIP PNRDPHFYVE MTFSQDVLCE CRPILETTKA ERRKTKGKRK RSRNSQTEEP HPHHHHHH.

Product Science Overview

Structure and Function

PlGF-1 is a glycoprotein that is primarily expressed in the placenta. It binds to the VEGF receptor-1 (VEGFR-1), also known as Flt-1, and modulates the activity of VEGF-A, another member of the VEGF family. This interaction enhances the angiogenic response, promoting the growth and development of new blood vessels .

Recombinant PlGF-1

Recombinant PlGF-1 is produced using genetic engineering techniques, where the PlGF-1 gene from mice is inserted into a suitable expression system, such as E. coli. This allows for the large-scale production of PlGF-1 for research and therapeutic purposes .

Applications in Research

Recombinant PlGF-1 is widely used in research to study its role in various physiological and pathological processes. It is particularly valuable in investigating the mechanisms of angiogenesis and the development of potential therapeutic strategies for diseases characterized by abnormal blood vessel growth .

Therapeutic Potential

The therapeutic potential of PlGF-1 lies in its ability to promote angiogenesis. This makes it a promising candidate for treating conditions such as ischemic heart disease, peripheral artery disease, and wound healing. Additionally, PlGF-1 has been explored as a potential target for anti-angiogenic therapies in cancer treatment .

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