PGAM1 Mouse, Active

Phosphoglycerate Mutase 1 Mouse Recombinant, Active
Cat. No.
BT12729
Source
Escherichia Coli.
Synonyms
Phosphoglycerate mutase 1, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B, Pgam1, Pgam-1, 2310050F24Rik.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PGAM1 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 278 amino acids (1-254) and having a molecular mass of 31.4kDa.
PGAM1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PGAM1, a member of the phosphoglycerate mutase family, plays a crucial role in glucose and 2,3-BPGA metabolism. It catalyzes the reversible conversion of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Existing as a dimeric enzyme, PGAM1 is composed of varying proportions of muscle (MM), brain (BB), and hybrid (MB) isozymes depending on the tissue. Mutations in the PGAM1 gene can result in muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X.
Description
Recombinant Mouse PGAM1, expressed in E.coli, is a non-glycosylated polypeptide chain. This monomeric protein consists of 278 amino acids (residues 1-254), with a molecular weight of 31.4kDa. It features a 24 amino acid His-tag fused at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PGAM1 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the PGAM1 solution should be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain protein integrity, repeated freeze-thaw cycles should be avoided.
Purity
The purity of PGAM1 is greater than 95% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of PGAM1 is measured to be greater than 150 units/mg. One unit of activity is defined as the amount of enzyme required to convert 1.0 µmole of 3-phosphoglycerate to 2-phosphoglycerate per minute at a pH of 7.6 and a temperature of 37°C.
Synonyms
Phosphoglycerate mutase 1, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B, Pgam1, Pgam-1, 2310050F24Rik.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK.

Product Science Overview

Structure and Expression

The recombinant mouse PGAM1 protein is typically expressed in Escherichia coli and is often tagged with a His tag for purification purposes . The protein is biologically active and has a high purity level, making it suitable for various biochemical assays and research applications . The specific activity of this recombinant enzyme is greater than 150 units/mg, where one unit will convert 1.0 μmole of 3-phosphoglycerate to 2-phosphoglycerate per minute at pH 7.6 at 37°C .

Biological Significance

PGAM1 is a key enzyme in the glycolytic pathway, which is a central metabolic pathway for energy production in cells. It is involved in the metabolism of glucose and 2,3-bisphosphoglycerate (2,3-BPGA), playing a critical role in cellular respiration and energy production .

Research Applications

Recombinant mouse PGAM1 is widely used in research to study its role in various biological processes and diseases. For instance, PGAM1 has been implicated in cancer metabolism and the remodeling of the tumor microenvironment. Studies have shown that PGAM1 promotes breast cancer progression by inducing immunosuppressive M2 macrophages . This makes it a potential target for cancer therapy, as inhibiting PGAM1 could reduce tumor growth and improve the efficacy of immunotherapy .

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