PGAM1 Mouse

PGAM1 belongs to the phosphoglycerate mutase family and is a dimeric enzyme. It exists in different isoforms, including a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) found in various tissues . The enzyme is essential for efficient energy production and is involved in the metabolism of glucose and 2,3-bisphosphoglycerate (2,3-BPGA) .

Recombinant mouse PGAM1 is a bioactive protein that has been engineered for research purposes. It is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant version often includes a His-tag at the N-terminus to facilitate purification and detection .

Recombinant PGAM1 is used in various biochemical and physiological studies to understand its role in metabolism and disease. For instance, it has been studied in the context of cancer metabolism, where it is known to promote tumor progression by inducing immunosuppressive M2 macrophages . This makes PGAM1 a potential target for cancer therapy, particularly in breast cancer .

For optimal stability, recombinant mouse PGAM1 should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain its activity .