Introduction
PGAM1, a member of the phosphoglycerate mutase family, plays a crucial role in glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism. This enzyme catalyzes the reversible conversion of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) within the glycolytic pathway. Existing as a dimer, PGAM1 comprises varying proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) depending on the tissue. Mutations in the PGAM1 gene can lead to muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X.
Physical Appearance
The product appears as a colorless solution that has undergone sterile filtration.
Formulation
The product is supplied at a concentration of 1mg/ml in a buffer solution containing PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), maintain the product at 4°C. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This PGAM1 antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, ICC/IF, and Flow cytometry, to ensure its specificity and reactivity. However, it is essential to note that optimal working dilutions may vary depending on the specific application. Therefore, users are advised to perform their own titrations to determine the optimal working concentration for their specific needs.
Synonyms
Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA.
Purification Method
PGAM1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human PGAM1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PGAM1 amino acids 1-254 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and l light chain.
