PEBP1 Mouse

Phosphatidylethanolamine Binding Protein 1 Mouse Recombinant
Cat. No.
BT6335
Source
Escherichia Coli.
Synonyms
Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PEBP1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-187 a.a) and having a molecular mass of 23.2kDa.
PEBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PEBP1 (Phosphatidylethanolamine binding protein 1) is a member of the phosphatidylethanolamine-binding protein family and acts as a serine protease inhibitor, inhibiting thrombin and neuropsin. It plays a crucial regulatory role in various protein kinase signaling pathways. The phosphorylation of PEBP1 by PKC triggers the release of Raf-1, subsequently activating MEK and ERK. PEBP1 is widely expressed in tissues and is implicated in regulating physiological processes such as membrane biosynthesis, spermatogenesis, neural development, and the suppression of metastasis. Notably, PEBP1 exhibits binding affinity for ATP, opioids, and phosphatidylethanolamine, with a lower affinity for phosphatidylinositol and phosphatidylcholine. It is suggested that PEBP1 may be involved in the functioning of presynaptic cholinergic neurons within the central nervous system (CNS). It is known to enhance choline acetyltransferase production, although not acetylcholinesterase. Additionally, PEBP1 potentially functions in sequestering toxic substances, including locostatin, which could have detrimental effects on cells. The loss of PEBP1 expression may serve as a significant prognostic marker in gastrointestinal stromal tumors. Moreover, differential expression of PEBP1 has been observed in the Wernicke's area of individuals with schizophrenia. PEBP1 also acts as an invasion suppressor protein in nasopharyngeal carcinoma.
Description
Recombinant PEBP1 protein, derived from mice and produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 210 amino acids (specifically, amino acids 1-187) and possesses a molecular mass of 23.2 kDa. The protein includes a 23-amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
The product appears as a sterile-filtered solution, devoid of color.
Formulation
The PEBP1 protein solution is provided at a concentration of 1 mg/ml. It is formulated in a solution of Phosphate Buffered Saline (PBS) at a pH of 7.4 and contains 10% glycerol.
Stability
For optimal storage, the product should be kept at 4°C if the entire vial is intended for use within a 2-4 week period. For extended storage, freezing at -20°C is recommended. To further ensure stability during long-term storage, the addition of a carrier protein (either 0.1% HSA or BSA) is advisable. It is important to minimize exposure to repeated freeze-thaw cycles.
Purity
The purity of the PEBP1 protein is determined to be greater than 95.0% based on SDS-PAGE analysis.
Synonyms
Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAADISQ WAGPLCLQEV DEPPQHALRV DYAGVTVDEL GKVLTPTQVM NRPSSISWDG LDPGKLYTLV LTDPDAPSRK DPKFREWHHF LVVNMKGNDI SSGTVLSDYV GSGPPSGTGL HRYVWLVYEQ EQPLSCDEPI LSNKSGDNRG KFKVETFRKK YNLGAPVAGT CYQAEWDDYV PKLYEQLSGK.

Product Science Overview

Discovery and Cloning

PEBP1 was first identified through a yeast two-hybrid screen as a protein that interacts with RAF1, a kinase involved in the MAPK signaling pathway . The protein inhibits the phosphorylation and activation of MEK by RAF1, thereby regulating the proliferation and differentiation of various cell types . The gene encoding PEBP1 has been cloned and characterized in multiple species, including humans, mice, and rats .

Structure and Expression

PEBP1 is a small protein with a molecular mass of approximately 23 kDa . It is highly conserved across species, with the human and bovine proteins sharing 95% identity . The protein is expressed in various tissues, with particularly high levels in the brain and testis . In the brain, PEBP1 is strongly expressed in the cell bodies of oligodendrocytes .

Functions and Significance

PEBP1 is involved in several critical cellular processes:

  • Cell Differentiation and Migration: PEBP1 modulates signaling pathways that control cell differentiation and migration .
  • Apoptosis and Cell Cycle: The protein plays a role in regulating apoptosis (programmed cell death) and the cell cycle .
  • Innate Immunity: PEBP1 is implicated in the innate immune response .
Role in Disease

PEBP1 has been studied for its potential role in various diseases, including cancer and neurodegenerative disorders. It has been proposed as a biomarker for Alzheimer’s disease due to its ability to suppress the Raf1-MAPK pathway . Additionally, alterations in PEBP1 expression have been associated with certain types of cancer .

Recombinant PEBP1

Recombinant PEBP1, particularly from mouse sources, is commonly used in research to study its functions and interactions. The recombinant protein is produced using various expression systems and is utilized in assays such as ELISA and Western blotting to investigate its role in different cellular processes .

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THE BIOTEK
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