PEBP1 Human

PEBP1, short for Phosphatidylethanolamine binding protein 1, is a multifaceted protein with a significant role in cellular processes. It belongs to a family of proteins known for their ability to bind phosphatidylethanolamine and acts as a serine protease inhibitor, regulating enzymes like thrombin and neuropsin. PEBP1 plays a crucial role in modulating various protein kinase signaling pathways. For instance, it interacts with PKC, leading to the release of Raf-1 and subsequent activation of MEK and ERK. Found in numerous tissues, PEBP1 is involved in regulating vital physiological processes such as membrane biosynthesis, spermatogenesis, neural development, and the suppression of metastasis. This protein exhibits binding affinity for molecules like ATP, opioids, and phosphatidylethanolamine, with a lower affinity for phosphatidylinositol and phosphatidylcholine. Additionally, it might contribute to the functioning of presynaptic cholinergic neurons within the central nervous system. While it enhances choline acetyltransferase production, it doesn't have the same effect on acetylcholinesterase. Notably, PEBP1 may also sequester potentially toxic compounds like locostatin, which could have detrimental cellular effects. Loss of PEBP1 expression can serve as a potential prognostic marker for Gastrointestinal stromal tumors. Moreover, differential expression of PEBP1 is observed in the Wernicke's Area of individuals with schizophrenia. Adding to its multifaceted nature, PEBP1 also functions as an invasion suppressor protein in nasopharyngeal carcinoma.

Recombinant human PEBP1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 187 amino acids (specifically, amino acids 1 to 187). With a molecular weight of 21 kDa, this protein is purified using proprietary chromatographic techniques.

The product appears as a sterile, colorless solution after filtration.

The PEBP1 protein is supplied in a solution containing 20mM Tris-HCl buffer at a pH of 8.0, 1mM DTT (dithiothreitol), and 10% glycerol.

For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freezing and thawing of the product should be avoided.

The purity of this product is determined to be greater than 95% using SDS-PAGE analysis.

Phosphatidylethanolamine-binding protein 1, Prostatic-binding protein, HCNPpp, Neuropolypeptide h3, Raf kinase inhibitor protein, PEBP-1, RKIP, PEBP1, PBP, PEBP, HCNP.

Escherichia Coli.

MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK.