PDPN P56F7AT Antibody

Podoplanin, also known by various synonyms such as Glycoprotein 36 (GP36), PA2.26 antigen, T1A, and others, is a small mucin-like type-1 transmembrane protein. It is typically expressed in various specialized cell types throughout the body. The Podoplanin clone P56F7AT is a monoclonal antibody derived from mouse and is specifically designed to target human Podoplanin.

Podoplanin plays a crucial role in various physiological processes due to its mucin-type character. It is expressed in lymphatic progenitor cells and later in lymphatic endothelial cells during development. Podoplanin is a specific marker for lymph vessel endothelial cells and is involved in cell adhesion, migration, and tube formation. Over-expression of Podoplanin significantly elevates endothelial cell adhesion, migration, and tube formation, while inhibition decreases cell adhesion in human dermal lymphatic endothelial cells .

Podoplanin is widely distributed in human tissues and is used as a specific marker for lymphatic endothelium in histopathology. Its expression is increased in nearly all human colon, rectum, and small intestine tumors. Additionally, Podoplanin may serve as a diagnostic marker to distinguish seminomas, which overexpress the protein, from embryonal carcinoma in testicular germ cell tumors .

The anti-human PDPN monoclonal antibody (mAb) is derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PDPN amino acids 99-207 purified from E. coli. The antibody belongs to the mouse IgG 1 heavy chain and k light chain subclass .

The PDPN antibody is purified from mouse ascitic fluids using protein-A affinity chromatography. It is formulated as a sterile filtered colorless solution containing 1mg/ml PBS (pH 7.4), 10% glycerol, and 0.02% sodium azide .

For optimal stability, the PDPN antibody should be stored at -20°C for up to 12 months or at 4°C for up to 1 month. It is important to prevent freeze-thaw cycles to maintain the antibody’s integrity .

The PDPN antibody has been tested for specificity and reactivity using various techniques, including ELISA, Western blot analysis, flow cytometry, and immunocytochemistry/immunofluorescence (ICC/IF). Each application should be titrated by the reagent to obtain optimal results .

ProSpec’s products, including the PDPN antibody, are intended for laboratory research use only. They are not to be used as drugs, agricultural or pesticidal products, food additives, or household chemicals .