PDPN Human
Description
PDPN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (99-207 a.a) and having a molecular mass of 13.4kDa.
PDPN is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
During mouse development, PDPN is expressed in lymphatic progenitor cells and subsequently in lymphatic endothelial cells. It serves as a specific marker for lymph vessel endothelial cells. Notably, over-expression of podoplanin significantly enhances endothelial cell adhesion, migration, and tube formation. Conversely, inhibiting Podoplanin expression reduces cell adhesion in human dermal lymphatic endothelial cells. In histopathology, podoplanin is utilized as a specific marker for lymphatic endothelium.
Increased podoplanin expression is observed in almost all human colon, rectum, and small intestine tumors. AGGRUS, potentially, can be used as a diagnostic marker to differentiate seminomas (which mostly overexpress the protein) from embryonal carcinoma in testicular germ cell tumors.
Recombinant Human PDPN, produced in E.Coli, is a single, non-glycosylated polypeptide chain. It consists of 130 amino acids (99-207 a.a), resulting in a molecular mass of 13.4kDa. The PDPN is fused to a 21 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Avoid repeated freeze-thaw cycles.
MGSSHHHHHH SSGLVPRGSH MASTGQPEDD TETTGLEGGV AMPGAEDDVV TPGTSEDRYK SGLTTLVATS VNSVTGIRIE DLPTSESTVH AQEQSPSATA SNVATSHSTE KVDGDTQTTV EKDGLSTVTL.
Product Science Overview
Podoplanin, also known as PDPN, is a small mucin-type transmembrane glycoprotein. It is highly conserved across species, including humans, mice, rats, dogs, and hamsters . Podoplanin is involved in various physiological and pathological processes, making it a significant molecule in biomedical research.
Podoplanin is a 36- to 43-kDa protein that is extensively expressed in different tissues and cells. These include lymphatic endothelial cells, type I alveolar cells, osteocytes, choroid plexus epithelial cells, glial cells, and stromal reticular cells in lymphoid organs . The protein’s structure includes a short cytoplasmic tail that interacts with proteins of the ezrin/radixin/moesin family, which function as cross-linkers between actin filaments and the plasma membrane .
Podoplanin plays a crucial role in several biological processes:
- Organ Development: It is essential for the development of the heart, lungs, and lymphatic system .
- Cell Motility and Tumorigenesis: Podoplanin is upregulated in tumor cells undergoing epithelial-mesenchymal transition, which is correlated with increased motility and metastasis .
- Immune System: It is involved in the biology of immune cells, including T cells and dendritic cells .
- Neurological Functions: Podoplanin is implicated in brain development, angiogenesis, tumors, ischemic stroke, and other neurological disorders .
Podoplanin is widely used as a marker for lymphatic endothelial cells and fibroblastic reticular cells of lymphoid organs. It is also used to identify lymphatics in the skin and tumor microenvironment . The protein’s ability to aggregate and activate CLEC-2-expressing platelets makes it a significant player in thrombosis and inflammation .
