PDPN Antibody

Podoplanin, Mouse Anti Human
Cat. No.
BT29345
Source
Synonyms
Podoplanin, Glycoprotein 36, PA2.26 antigen, T1A, GP36, GP40, Gp38, OTS8, T1A2, HT1A-1, PA2.26, T1-alpha, PDPN.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Podoplanin, a small mucin-like type-1 transmembrane protein, is expressed in various specialized cell types. As a type-I integral membrane glycoprotein, it's found in diverse human tissues. Its mucin-type character contributes to its physiological function. In other species, the homologous protein acts as a differentiation antigen and influenza-virus receptor. During mouse development, PDPN is expressed in lymphatic progenitor cells and later in lymphatic endothelial cells, making it a specific marker for these cells. Overexpressing podoplanin significantly increases endothelial cell adhesion, migration, and tube formation, while inhibiting it decreases cell adhesion in human dermal lymphatic endothelial cells. Therefore, it serves as a specific marker for lymphatic endothelium in histopathology. Notably, podoplanin expression is elevated in most human colon, rectum, and small intestine tumors. AGGRUS, potentially, can differentiate seminomas (which generally overexpress the protein) from embryonal carcinoma in testicular germ cell tumors.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be stored at 4°C. For extended storage, it is recommended to store the antibody at -20°C. Repeated freezing and thawing of the antibody should be avoided.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
The PDPN antibody has undergone testing using both ELISA and Western blot analysis to confirm its specificity and reactivity. However, as specific experimental conditions may vary, it is recommended to titrate the antibody for each application to determine the optimal working concentration. For Western blot analysis, a dilution range of 1:500 to 1:1000 is suggested, with an initial dilution of 1:500 being a suitable starting point.
Synonyms
Podoplanin, Glycoprotein 36, PA2.26 antigen, T1A, GP36, GP40, Gp38, OTS8, T1A2, HT1A-1, PA2.26, T1-alpha, PDPN.
Purification Method
PDPN antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P5E2AT.
Immunogen
Anti-human PDPN mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PDPN amino acids 1-206 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

Podoplanin is a type I transmembrane glycoprotein that is widely expressed in various tissues and cell types. It is known by several other names, including gp36, Aggrus, PA2.26 antigen, and T1 alpha. Podoplanin plays a crucial role in the development of the lymphatic system, cell migration, and tumor progression. The mouse anti-human podoplanin antibody is a monoclonal antibody that specifically targets human podoplanin, making it a valuable tool in research and diagnostic applications.

Structure and Function of Podoplanin

Podoplanin is a 140 amino acid protein with a molecular weight of approximately 36 kDa. It is characterized by its single-pass transmembrane domain and an extracellular domain that is heavily glycosylated. The extracellular domain contains three platelet aggregation-stimulating (PLAG) domains, which are responsible for its interaction with the C-type lectin-like receptor 2 (CLEC-2) on platelets . This interaction induces platelet aggregation and is crucial for maintaining the integrity of the lymphatic system.

Podoplanin is involved in various biological processes, including:

  • Lymphangiogenesis: Podoplanin is strongly expressed on lymphatic endothelial cells and is essential for the formation and maintenance of the lymphatic vasculature .
  • Cell Migration and Invasion: Podoplanin promotes cell migration and invasion by reorganizing the actin cytoskeleton and enhancing cellular adhesion .
  • Tumor Progression: Podoplanin expression is upregulated in several types of tumors, where it contributes to tumor cell migration, invasion, and metastasis .
Mouse Anti-Human Podoplanin Antibody

The mouse anti-human podoplanin antibody is a monoclonal antibody that specifically binds to human podoplanin. It is produced by immunizing mice with the human podoplanin ectodomain expressed in Escherichia coli. The antibody is then purified from the mouse serum using affinity chromatography.

Applications

The mouse anti-human podoplanin antibody is widely used in various research and diagnostic applications, including:

  • Immunohistochemistry (IHC): This antibody is used to detect podoplanin expression in tissue sections, allowing researchers to study its distribution and localization in different tissues .
  • Western Blotting (WB): The antibody is used to detect podoplanin in protein extracts from cells and tissues, providing information about its expression levels .
  • Flow Cytometry (FC): The antibody is used to analyze podoplanin expression on the surface of cells, enabling the identification and characterization of podoplanin-expressing cell populations .
  • Enzyme-Linked Immunosorbent Assay (ELISA): The antibody is used in ELISA to quantify podoplanin levels in biological samples .
Specificity and Validation

The mouse anti-human podoplanin antibody has been extensively validated for its specificity and performance. It has been shown to specifically bind to human podoplanin without cross-reacting with other proteins. The antibody’s specificity has been confirmed through various techniques, including relative expression analysis and knockdown experiments .

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THE BIOTEK
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