Prolactin Ovine, His

Recombinant Ovine Prolactin is produced using Escherichia coli (E. coli) as the expression system . The recombinant protein is a single, non-glycosylated polypeptide chain that is fused to a 6x His Tag at the C-terminus . This His Tag facilitates the purification process through affinity chromatography, allowing for a high degree of purity .

The recombinant ovine prolactin is formulated as a sterile, filtered, colorless solution. It is typically stored in a buffer solution of 1X PBS (phosphate-buffered saline) with a pH of 7.2 and 50% glycerol . The protein is more than 90% pure as determined by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) .

For short-term storage (2-4 weeks), the recombinant protein can be kept at 4°C. For longer periods, it should be stored frozen at -20°C. To ensure stability during long-term storage, it is recommended to add a carrier protein such as 0.1% HSA (human serum albumin) or BSA (bovine serum albumin) . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .

Recombinant ovine prolactin is used extensively in laboratory research. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical . Its applications include studies on lactation, breast cancer, reproductive function, and immunoregulation .