OPG Human, His
(b) Analysis by SDS-PAGE.
Description
The OPG is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Osteoprotegerin (OPG), also known as Tumor Necrosis Factor Receptor Superfamily Member 11B (TNFRSF11B), is a crucial protein involved in bone metabolism. It acts as a decoy receptor for the receptor activator of nuclear factor kappa-Β ligand (RANKL), inhibiting osteoclast differentiation and activity, thereby preventing bone resorption. The recombinant form of this protein, tagged with a His-tag, is widely used in research to study its function and potential therapeutic applications.
The human recombinant osteoprotegerin protein is typically expressed in insect cells using a baculovirus expression system. This method ensures high yield and proper post-translational modifications, which are essential for the protein’s biological activity. The His-tag, a sequence of histidine residues, is added to the C-terminus of the protein to facilitate purification through affinity chromatography .
Osteoprotegerin functions by binding to RANKL, preventing it from interacting with its receptor RANK on the surface of osteoclast precursors. This interaction is crucial for osteoclast differentiation and activation. By inhibiting this pathway, OPG plays a significant role in maintaining bone density and preventing excessive bone resorption .
Recombinant human osteoprotegerin with a His-tag is used extensively in research to:
The production of recombinant OPG involves cloning the human OPG gene into a baculovirus vector, which is then used to infect insect cells. The expressed protein is harvested and purified using nickel affinity chromatography, exploiting the His-tag for selective binding. The purified protein is then subjected to further quality control measures, including SDS-PAGE and endotoxin testing, to ensure its purity and biological activity .
