OPG Mouse
Description
OPG is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Osteoprotegerin is produced by osteoblasts and acts as a decoy receptor for the receptor activator of nuclear factor kappa-Β ligand (RANKL). By binding to RANKL, OPG prevents it from interacting with its receptor, RANK, on the surface of osteoclast precursors. This inhibition of the RANKL-RANK interaction reduces the formation, function, and survival of osteoclasts, thereby decreasing bone resorption and promoting bone formation .
Recombinant mouse osteoprotegerin is produced using various expression systems, such as HEK293 cells or mouse myeloma cell lines (NS0). The recombinant protein is typically purified to a high degree of purity (>95%) and is often used in research to study bone metabolism and related diseases .
Key Characteristics:
- Molecular Mass: The predicted molecular mass of recombinant mouse OPG is approximately 70.2 kDa, but it may appear as 85-95 kDa under reducing conditions in SDS-PAGE due to glycosylation .
- Activity: Recombinant mouse OPG is biologically active and can inhibit TRAIL-mediated cytotoxicity in certain cell lines .
- Formulation: It is usually lyophilized from a sterile PBS solution and can be reconstituted in PBS for use in various applications .
Osteoprotegerin plays a significant role in maintaining bone homeostasis. The balance between RANKL and OPG is critical for bone remodeling. An imbalance, such as reduced OPG levels or increased RANKL levels, can lead to bone diseases like osteoporosis and rheumatoid arthritis. Studies have also suggested that OPG and its ligand are involved in lymph-node organogenesis and vascular calcification .
