NME2 Rat

Non-Metastatic Cells 2 Rat Recombinant
Cat. No.
BT11804
Source
Escherichia Coli.
Synonyms
Nucleoside diphosphate kinase B, NDK B, NDP kinase B, Histidine protein kinase NDKB, P18.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NME2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 175 amino acids (1-152 a.a) and having a molecular mass of 19.7kDa.
NME2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NME2 plays a crucial role in the synthesis of nucleoside triphosphates, excluding ATP. It negatively regulates Rho activity through interaction with AKAP13/LBC. Additionally, NME2 functions as a transcriptional activator of the MYC gene and exhibits non-specific DNA binding. As a heterodimeric enzyme, NME2 acts as a nucleoside diphosphate kinase. Both NME1 and NME2 consist of 152 amino acids, representing the A and B polypeptide chains of the NM23 enzyme, respectively. Notably, NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, while NM23 participates in signal transduction regulation by forming complexes with G proteins, thereby influencing the activation or inactivation of developmental pathways.
Description
Recombinant Rat NME2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 175 amino acids (1-152 a.a.). With a molecular weight of 19.7 kDa, NME2 is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
NME2 protein solution at a concentration of 1 mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 90.0% as determined by SDS-PAGE analysis.
Synonyms
Nucleoside diphosphate kinase B, NDK B, NDP kinase B, Histidine protein kinase NDKB, P18.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMANLERT FIAIKPDGVQ RGLVGEIIKR FEQKGFRLVA MKFLRASEEH LKQHYIDLKD RPFFPGLVKY MNSGPVVAMV WEGLNVVKTG RVMLGETNPA DSKPGTIRGD FCIQVGRNII HGSDSVESAE KEIGLWFKPE ELIDYKSCAH DWVYE.

Product Science Overview

Structure and Function

NME2 is a homodimeric enzyme that catalyzes the transfer of phosphate groups from nucleoside triphosphates to nucleoside diphosphates. This reaction is essential for maintaining the balance of nucleoside triphosphates within the cell. The protein is composed of 152 amino acids and has a molecular weight of approximately 17 kDa.

The primary function of NME2 is to regulate the levels of nucleoside triphosphates, which are necessary for DNA and RNA synthesis. Additionally, NME2 has been implicated in various cellular processes, including:

  • Cell Proliferation: NME2 promotes cell division and growth by ensuring a steady supply of nucleotides for DNA replication.
  • Differentiation: The protein plays a role in the differentiation of cells into specialized types.
  • Apoptosis: NME2 is involved in programmed cell death, helping to eliminate damaged or unwanted cells.
Role in Cancer

NME2 has been studied extensively in the context of cancer. It is known to act as a metastasis suppressor, meaning it helps prevent the spread of cancer cells to other parts of the body. In various cancer types, including lung, breast, and colorectal cancers, reduced expression of NME2 has been associated with increased metastatic potential and poor prognosis.

Research has shown that NME2 can inhibit the migration and invasion of cancer cells by modulating the activity of various signaling pathways. For example, NME2 has been found to interact with proteins involved in the Wnt/β-catenin signaling pathway, which is crucial for cell proliferation and migration.

Applications in Research

The recombinant form of NME2 from rats is widely used in research to study its biochemical properties and biological functions. Some of the key applications include:

  • Cancer Research: Investigating the role of NME2 in tumor progression and metastasis.
  • Cell Biology: Studying the protein’s involvement in cell proliferation, differentiation, and apoptosis.
  • Drug Development: Screening for potential therapeutic agents that can modulate the activity of NME2.

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