NME2 Human

Non-Metastatic Cells 2 Human Recombinant
Cat. No.
BT19475
Source
Escherichia Coli.
Synonyms
Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B, EC 2.7.4.6, NDP kinase B, C-myc purine-binding transcription factor PUF, NDK B, NME2, puf, MGC111212.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NME2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.2kDa. The NME2 is purified by conventional chromatography.

Product Specs

Introduction
NME2 plays a crucial role in the synthesis of nucleoside triphosphates, excluding ATP. It negatively regulates Rho activity through interaction with AKAP13/LBC and functions as a transcriptional activator of the MYC gene. NME2 exhibits non-specific DNA binding. As a heterodimeric enzyme, it acts as a nucleoside diphosphate kinase. Both NME1 and NME2 consist of 152 amino acids, representing the A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit found in human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, while NM23 participates in signal transduction regulation by forming complexes with G proteins, thereby activating or inactivating developmental pathways.
Description
Recombinant human NME2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 152 amino acids (1-152 a.a.) with a molecular weight of 17.2 kDa. Purification of NME2 is achieved through conventional chromatography techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The NME2 protein solution is formulated in 20mM Tris-HCl (pH 8), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure long-term stability during storage, adding a carrier protein such as 0.1% HSA or BSA is advisable. It's important to minimize repeated freeze-thaw cycles.
Purity
The purity of the NME2 protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B, EC 2.7.4.6, NDP kinase B, C-myc purine-binding transcription factor PUF, NDK B, NME2, puf, MGC111212.
Source
Escherichia Coli.
Amino Acid Sequence
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE.

Product Science Overview

Introduction

Non-Metastatic Cells 2 (NME2) is a protein encoded by the NME2 gene in humans. It is part of the NME/NM23 family, which is known for its role in inhibiting metastasis in various types of cancer. The recombinant form of this protein is produced through genetic engineering techniques, allowing for its use in research and therapeutic applications.

Structure and Function

NME2 is a nucleoside diphosphate kinase (NDPK) that plays a crucial role in the synthesis of nucleoside triphosphates other than ATP. It is involved in various cellular processes, including:

  • Cell proliferation
  • Differentiation
  • Apoptosis

The protein functions as a hexamer and is known to interact with other proteins to exert its effects. Its role in inhibiting metastasis is particularly significant, as it helps prevent the spread of cancer cells to other parts of the body.

Mechanism of Action

The anti-metastatic properties of NME2 are attributed to several mechanisms:

  1. Regulation of Gene Expression: NME2 influences the expression of genes involved in cell adhesion, motility, and invasion.
  2. Signal Transduction: It participates in signaling pathways that control cell growth and differentiation.
  3. DNA Repair: NME2 is involved in the repair of damaged DNA, maintaining genomic stability.
Clinical Significance

NME2 has been studied extensively for its potential therapeutic applications. Its ability to inhibit metastasis makes it a promising candidate for cancer treatment. Research has shown that higher levels of NME2 are associated with better prognosis in cancer patients.

Recombinant Production

The recombinant form of NME2 is produced using bacterial or mammalian expression systems. This involves inserting the NME2 gene into a plasmid vector, which is then introduced into host cells. The host cells express the protein, which can be purified and used for various applications, including:

  • Cancer research
  • Drug development
  • Therapeutic interventions

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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