NT 3 Mouse
(b) Analysis by SDS-PAGE.
Description
Neurotrophin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13.6kDa.
The NT-3 is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Mouse Neurotrophin-3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 119 amino acids. With a molecular weight of 13.6 kDa, this purified protein is obtained through proprietary chromatographic techniques.
Biological activity, assessed by the dose-dependent proliferation of BaF3 cells transfected with the TrkB receptor, typically falls within the range of 1-10 ng/ml. This corresponds to a specific activity of 100,000-1,000,000 units/mg.
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Product Science Overview
Neurotrophin-3 (NT-3) is a member of the neurotrophin family, which includes nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-4 (NT-4). These proteins play crucial roles in the development, maintenance, and function of the nervous system by promoting the survival, differentiation, and growth of neurons.
NT-3 is a protein that is encoded by the NTF3 gene. The protein is initially synthesized as a precursor molecule, which is then cleaved to produce the mature, biologically active form. The mature NT-3 protein consists of 119 amino acids and has a molecular weight of approximately 13.6 kDa .
NT-3 exerts its effects by binding to specific receptors on the surface of target cells. The primary receptor for NT-3 is TrkC (tropomyosin receptor kinase C), although it can also interact with TrkA and TrkB receptors to a lesser extent. Upon binding to its receptor, NT-3 activates intracellular signaling pathways that promote neuronal survival, differentiation, and growth .
NT-3 is known to selectively promote the differentiation and survival of specific neuronal subpopulations in both the central and peripheral nervous systems. It has been shown to mediate neurite outgrowth and induce phosphorylation of ERK1/2 MAPK in TrkC-transfected cells . These activities are essential for the proper development and function of the nervous system.
Recombinant NT-3 is produced using various expression systems, including E. coli. The recombinant protein is typically purified to high levels of purity (>95%) and is free from endotoxins, making it suitable for research applications . The recombinant mouse NT-3 protein is used in various experimental settings to study its effects on neuronal cells and to investigate potential therapeutic applications for neurodegenerative diseases and nerve injuries.
Recombinant NT-3 has been widely used in research to study its role in neuronal development and function. It has been employed in cell culture experiments to promote neurite outgrowth and to investigate the signaling pathways activated by NT-3. Additionally, recombinant NT-3 has potential therapeutic applications in the treatment of neurodegenerative diseases, such as Alzheimer’s disease, and in promoting nerve regeneration following injury .
