NT 3 Human

NT-3 is a protein that is highly conserved across species, with identical amino acid sequences in humans, mice, and pigs . It exists as a monomer with a molecular weight of approximately 13.6 kDa or as a dimer with a molecular weight of 27.2 kDa . The protein is involved in the development and maintenance of neuronal populations and promotes the differentiation of neural crest-derived sensory and sympathetic neurons .

NT-3 exerts its effects by binding to and activating specific receptors on the surface of target cells. It primarily interacts with the TrkC receptor but can also bind to TrkA and TrkB receptors . Upon binding, these receptors activate several intracellular signaling pathways, including the Ras/MAPK pathway, the PI3 kinase/Akt pathway, and the PLC-γ1/PKC pathway . These pathways are involved in promoting neuronal differentiation, survival, growth, and synaptic plasticity .

NT-3 is expressed in various cell types, including neuronal cells, eosinophils, and melanocytes . It is essential for the development of peripheral sensory neurons and is required for the proper functioning of the nervous system . Knock-out studies in mice have shown that the absence of NT-3 leads to movement and posture deficiencies due to proprioception defects and loss of neurons in spinal and cephalic sensory ganglia .

Recombinant human NT-3 (hNT-3) is produced using recombinant DNA technology, typically in bacterial systems such as E. coli . The recombinant protein is purified to high levels of purity, often greater than 95% . It is used in various research applications to study its effects on neuronal cells and to understand its role in the nervous system.

Recombinant hNT-3 is supplied in a lyophilized form and can be reconstituted with sterile water or buffer solutions . It is stable at -20°C for long-term storage and can be used in various experimental setups to study its biological activity .