NAP1L4 Human

Nucleosome Assembly Protein 1-Like 4 Human Recombinant
Cat. No.
BT20968
Source
Escherichia Coli.
Synonyms
Nucleosome assembly protein 1-like 4, Nucleosome assembly protein 2, NAP-2, NAP1L4, NAP2, hNAP2, NAP1L4b, NAP2L.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NAP1L4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-375) and having a molecular mass of 45.2 kDa.
NAP1L4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Nucleosome Assembly Protein 1-Like 4 (NAP1L4) belongs to the nucleosome assembly protein (NAP) family and interacts with both core and linker histones. Functioning as a histone chaperone, NAP1L4 shuttles between the cytoplasm and nucleus, playing a role in modulating transcriptional activation by target nuclear receptors. Notably, NAP1L4 is situated near the imprinted gene domain of 11p15.5, a region recognized for its tumor-suppressor genes.
Description
Recombinant human NAP1L4, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 398 amino acids (residues 1-375) with a molecular weight of 45.2 kDa. An N-terminal 23 amino acid His-tag is fused to NAP1L4, and purification is achieved through proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The NAP1L4 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
Purity is determined to be greater than 90.0% as assessed by SDS-PAGE analysis.
Synonyms
Nucleosome assembly protein 1-like 4, Nucleosome assembly protein 2, NAP-2, NAP1L4, NAP2, hNAP2, NAP1L4b, NAP2L.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADHSFS DGVPSDSVEA AKNASNTEKL TDQVMQNPRV LAALQERLDN VPHTPSSYIE TLPKAVKRRI NALKQLQVRC AHIEAKFYEE VHDLERKYAA LYQPLFDKRR EFITGDVEPT DAESEWHSEN EEEEKLAGDM KSKVVVTEKA AATAEEPDPK GIPEFWFTIF RNVDMLSELV QEYDEPILKH LQDIKVKFSD PGQPMSFVLE FHFEPNDYFT NSVLTKTYKM KSEPDKADPF SFEGPEIVDC DGCTIDWKKG KNVTVKTIKK KQKHKGRGTV RTITKQVPNE SFFNFFNPLK ASGDGESLDE DSEFTLASDF EIGHFFRERI VPRAVLYFTG EAIEDDDNFE EGEEGEEEEL EGDEEGEDED DAEINPKV.

Product Science Overview

Gene and Protein Structure

The NAP1L4 gene consists of 14 exons and spans approximately 30.5 kb . The gene encodes a highly acidic protein of 375 amino acids, which includes a nuclear localization motif and two clusters of highly acidic residues . This structure allows NAP1L4 to interact with both core and linker histones, facilitating the transfer of histones onto DNA templates .

Function and Mechanism

NAP1L4 acts as a histone chaperone, shuttling between the cytoplasm and nucleus . It assists in the assembly of nucleosomes by depositing preformed tetramers of histones H3 and H4 onto DNA before adding tetramers of histones H2A and H2B . This process is essential for the regulation of gene expression at the level of DNA packaging .

Clinical Significance

The NAP1L4 gene is located near an important tumor-suppressor gene region and has been associated with several disorders, including Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast . Alterations in this region can lead to various genetic disorders and malignancies .

Research and Applications

Recombinant NAP1L4 protein is used in various research applications to study chromatin dynamics, gene expression, and the role of histone chaperones in nucleosome assembly . Understanding the function and mechanism of NAP1L4 can provide insights into the regulation of gene expression and the development of cancer and other genetic disorders.

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NAP1L1 Human
Nucleosome Assembly Protein 1-Like 1 Human Recombinant
Cat. No.
BT20886
Source
E.coli.
THE BIOTEK
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