NAP1L1 Human

Nucleosome Assembly Protein 1-Like 1 Human Recombinant
Cat. No.
BT20886
Source
E.coli.
Synonyms
Nucleosome assembly protein 1-like 1, NRP, hNRP, NAP1L, NAP1, NAP-1-related protein, HSP22-like protein interacting protein, MGC23410, MGC8688.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NAP1L1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 408 amino acids (1-388 a.a.) and having a molecular mass of 47.2kDa.
NAP1L1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NAP1L1, a member of the nucleosome assembly protein family, participates in DNA replication, chromatin formation modulation, and cell proliferation regulation.
Description
Recombinant human NAP1L1, produced in E. coli, is a non-glycosylated polypeptide chain (408 amino acids, 1-388 a.a.) with a molecular weight of 47.2 kDa. It includes a 20 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The NAP1L1 protein solution (1 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Nucleosome assembly protein 1-like 1, NRP, hNRP, NAP1L, NAP1, NAP-1-related protein, HSP22-like protein interacting protein, MGC23410, MGC8688.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MADIDNKEQS ELDQDLDDVE EVEEEETGEE TKLKARQLTV QMMQNPQILA ALQERLDGLV ETPTGYIESL PRVVKRRVNA LKNLQVKCAQ IEAKFYEEVH DLERKYAVLY QPLFDKRFEI INAIYEPTEE ECEWKPDEED EISEELKEKA KIEDEKKDEE KEDPKGIPEF WLTVFKNVDL LSDMVQEHDE PILKHLKDIK VKFSDAGQPM SFVLEFHFEP NEYFTNEVLT KTYRMRSEPD DSDPFSFDGP EIMGCTGCQI DWKKGKNVTL KTIKKKQKHK GRGTVRTVTK TVSNDSFFNF FAPPEVPESG DLDDDAEAIL AADFEIGHFL RERIIPRSVL YFTGEAIEDD DDDYDEEGEE ADEEGEEEGD EENDPDYDPK KDQNPAEC.

Product Science Overview

Gene and Protein Structure

The NAP1L1 gene is located on chromosome 12 in humans and encodes a protein that is highly conserved across different species . The protein consists of several domains that facilitate its interaction with histones and other chromatin-associated proteins. These domains include:

  • N-terminal domain: Involved in binding to histones H2A-H2B.
  • C-terminal acidic domain: Plays a role in chromatin remodeling and nucleosome assembly.
  • Earmuff domain: Important for the interaction with histones and other proteins involved in chromatin dynamics .
Biological Functions

NAP1L1 functions primarily as a histone chaperone, facilitating the proper assembly and disassembly of nucleosomes. This activity is essential for maintaining chromatin structure and regulating gene expression. Some of the key functions of NAP1L1 include:

  • Nucleosome Assembly: NAP1L1 assists in the deposition of histones H2A and H2B onto DNA, forming nucleosomes .
  • DNA Repair: It enhances chromatin remodeling, which is crucial for DNA repair mechanisms such as transcription-coupled nucleotide excision repair and homologous recombination .
  • Cell Proliferation: NAP1L1 is involved in modulating chromatin formation, thereby contributing to the regulation of cell proliferation .
Expression Patterns and Tissue Distribution

NAP1L1 is ubiquitously expressed in various tissues, with higher expression levels observed in rapidly proliferating cells. Its expression is tightly regulated during the cell cycle, ensuring proper chromatin dynamics during DNA replication and cell division .

Regulatory Mechanisms

The activity of NAP1L1 is regulated through various post-translational modifications, including phosphorylation and acetylation. These modifications influence its interaction with histones and other chromatin-associated proteins, thereby modulating its function in nucleosome assembly and chromatin remodeling .

Clinical Significance

Mutations or dysregulation of NAP1L1 have been associated with several diseases, including certain cancers and viral infections. For instance, NAP1L1 has been implicated in the reactivation of Epstein-Barr virus in epithelial cells . Additionally, its role in cell proliferation and DNA repair makes it a potential target for therapeutic interventions in cancer treatment .

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Cat. No.
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Source
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