NANOG-TAT Human
Sterile Filtered solution.
Greater than 95.0% as determined by SDS-PAGE analysis.
Description
NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 304 amino acids and c-terminal 13 amino acid TAT peptide having a molecular mass of 36.1kDa.
The NANOG is purified by proprietary chromatographic techniques.
Product Specs
VDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDVGGYGRK KRRQRRR
Product Science Overview
NANOG is a homeobox transcription factor that plays a crucial role in the self-renewal of embryonic stem cells and the maintenance of pluripotency . It works in concert with other key transcription factors such as OCT4 and SOX2 to prevent differentiation and maintain the stem cell state . NANOG is essential during the early stages of embryogenesis and is involved in the formation of the epiblast, a structure that gives rise to the embryo proper .
The TAT peptide, derived from the HIV-1 virus, is known for its ability to transduce across cell membranes. This property makes it an effective delivery vehicle for various molecules, including proteins, into cells . When fused with NANOG, the TAT peptide facilitates the efficient delivery of the NANOG protein into cells, enhancing its biological activity and potential therapeutic applications .
Recombinant human NANOG-TAT is typically produced in Escherichia coli (E. coli) expression systems. The protein is synthesized as a single chain, non-glycosylated polypeptide consisting of 318 amino acids . The molecular mass of NANOG-TAT is approximately 36.2 kDa, as determined by SDS-PAGE analysis . The production process involves the fusion of the NANOG cDNA sequence with the TAT peptide sequence, followed by purification using chromatographic techniques .
NANOG-TAT retains the biological activity of the native NANOG protein, making it a valuable tool for research in stem cell biology and regenerative medicine . It has been shown to enhance cell migration and downregulate the expression of certain cell cycle inhibitors, such as p27, in cancer cells . This highlights its potential role as a therapeutic target for various malignancies, including cervical cancer .
