NANOG Antibody

Homeobox Protein NANOG, Mouse Anti Human
Cat. No.
BT27040
Source
Synonyms
NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
Appearance
Purity
Usage
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Description

Product Specs

Introduction
NANOG is a homeobox transcription factor with multiple domains. Its primary function is to maintain the pluripotency of stem cells by keeping them in an undifferentiated state. NANOG expression works against the signals that promote differentiation, such as those induced by LIF, Stat3, and BMP. A decrease in NANOG expression is a prerequisite for cell differentiation to begin. The regulation of NANOG expression is influenced by several factors, including transcription factors like Oct4, SOX2, FoxD3, Tcf3, and the tumor suppressor protein p53.
Formulation
The antibody solution has a concentration of 1mg/ml and is prepared in a buffer consisting of PBS at pH 7.4 with 0.1% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For longer storage durations, it is recommended to store the antibody at -20°C. Repeated freezing and thawing of the antibody should be avoided.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
The NANOG antibody has undergone rigorous testing in various applications, including ELISA, Western blot, and immunohistochemistry, to ensure its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application. For Western blot analysis, a dilution range of 1:500 to 1:1,000 is recommended, with a starting dilution of 1:500. For immunohistochemistry, a dilution range of 1:50 to 1:100 is recommended, with a starting dilution of 1:50.
Synonyms
NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
Purification Method
NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P5A10AT.
Immunogen
Anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Introduction

Homeobox protein NANOG is a transcription factor that plays a crucial role in maintaining the pluripotency and self-renewal of embryonic stem cells (ESCs). The name “NANOG” is derived from Tír na nÓg, the mythical Celtic land of eternal youth . This protein is encoded by the NANOG gene in humans and has been associated with various types of cancer .

Structure and Function

The human NANOG protein consists of 305 amino acids and possesses three functional domains: the N-terminal domain, the C-terminal domain, and the conserved homeodomain motif . NANOG functions by suppressing cell determination factors, thereby helping ESCs maintain their undifferentiated state .

Role in Stem Cell Research

NANOG is a member of the homeobox family of DNA-binding transcription factors. It has been shown to maintain ESC self-renewal independently of leukemia inhibitory factor (LIF)/Stat3 . NANOG mRNA is present in pluripotent mouse and human cell lines but is absent from differentiated cells . Functionally, NANOG works together with other key pluripotent factors such as Oct4, Sox2, and Lin28 to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells .

Mouse Anti Human NANOG Antibody

The mouse anti-human NANOG antibody is a monoclonal antibody derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E. coli . This antibody is used in various applications, including Western Blot (WB) and ELISA, and shows reactivity with human and mouse samples .

Applications and Importance

The NANOG antibody is widely used in stem cell research to demonstrate the pluripotency of ESCs and iPS cells . It is also used in cancer research due to its association with various types of cancer . The antibody can recognize two isoforms of NANOG with molecular weights of 34.4 kDa and 31.9 kDa .

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