NANOG Human, His

Nanog is a multidomain protein that contains a homeodomain responsible for DNA binding. This homeodomain allows Nanog to regulate the expression of genes involved in maintaining pluripotency and preventing differentiation. Nanog expression counteracts differentiation-promoting signals induced by extrinsic factors such as LIF (Leukemia Inhibitory Factor), Stat3, and BMP (Bone Morphogenetic Protein) .

Recombinant human Nanog with a His tag is produced using recombinant DNA technology. The His tag, a sequence of histidine residues, is added to the N-terminus of the Nanog protein to facilitate purification and detection. This recombinant form is typically expressed in Escherichia coli (E. coli) and purified using conventional chromatographic techniques .

Key Features:

• Source: E. coli
• Amino Acid Sequence: The recombinant protein contains 174 amino acids, including the His tag, with a molecular mass of approximately 19.6 kDa .
• Purity: Greater than 90% as determined by SDS-PAGE .
• Formulation: Supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, and 20% glycerol .

Recombinant Nanog (Human, His Tag) is widely used in research to study the mechanisms of stem cell pluripotency and differentiation. It is also used in various assays to investigate the role of Nanog in cellular processes and to develop potential therapeutic applications targeting stem cell regulation.

The recombinant Nanog protein should be stored at 4°C for short-term use (within 2-4 weeks) and at -20°C for long-term storage. It is recommended to avoid multiple freeze-thaw cycles to maintain protein stability .