Myostatin Human

Myostatin Human Recombinant
Cat. No.
BT23803
Source
Escherichia Coli.
Synonyms
GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Myostatin Human Recombinant produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 109 amino acids and having a total molecular mass of 24814 Dalton.
The GDF-8 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth differentiation factor 8 (GDF8), also known as myostatin, is a protein that belongs to the transforming growth factor beta (TGF-β) superfamily. This family of proteins plays crucial roles in regulating cell growth and differentiation in various tissues, both during embryonic development and in adulthood. GDF8 is synthesized as a precursor protein that undergoes proteolytic cleavage to generate its active form. The mature GDF8 protein is characterized by a structure containing seven conserved cysteine residues, which are essential for its biological activity. GDF8 is primarily expressed in skeletal muscle tissue, where it acts as a negative regulator of muscle growth.
Description
Recombinant human myostatin is a protein produced in E. coli. It is a homodimer, meaning it consists of two identical polypeptide chains. Each chain contains 109 amino acids, making a total of 218 amino acids in the myostatin molecule. The molecular mass of the myostatin homodimer is 24,814 Daltons. This myostatin product is non-glycosylated, meaning it does not have sugar molecules attached. It is purified using advanced chromatographic techniques to ensure high purity.
Physical Appearance
White powder, freeze-dried and sterilized by filtration.
Formulation
Freeze-dried from a 1 mg/ml solution without any additional substances.
Solubility
To reconstitute the freeze-dried myostatin, it is recommended to dissolve it in sterile 20 mM hydrochloric acid (HCl) at a concentration of 0.1 mg/ml. This solution can then be diluted further using other aqueous solutions as needed.
Stability
Lyophilized Myostatin remains stable for up to 3 weeks at room temperature but should ideally be stored in a dry environment at temperatures below -18°C. After reconstitution, Myostatin should be kept at 4°C for short-term storage (2-7 days) and frozen below -18°C for longer periods. Adding a carrier protein like HSA or BSA at a concentration of 0.1% is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of this Myostatin is over 95%, as determined by two methods: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of this Myostatin is measured by its ability to inhibit the growth of MPC-11 cells. The half-maximal effective concentration (ED50) is less than 20 ng/ml. This corresponds to a specific activity of 50,000 units per milligram of protein.
Protein Content
Two independent methods were employed to quantify the protein content: 1) UV spectroscopy at a wavelength of 280 nm, utilizing an extinction coefficient of 1.55 for a 0.1% (1 mg/ml) solution, as calculated using the PC GENE software for protein sequence analysis; 2) RP-HPLC, employing a standardized Myostatin solution as a reference.
Synonyms
GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.

Product Science Overview

Discovery and Function

Myostatin was first identified in 1997 by researchers studying muscle development in mice. It is produced and released by myocytes (muscle cells) and acts on muscle cells to inhibit muscle growth. This inhibitory effect is essential for preventing excessive muscle growth and maintaining muscle homeostasis .

Mechanism of Action

Myostatin functions by binding to the activin type II receptor on the surface of muscle cells. This binding initiates a signaling cascade that ultimately leads to the inhibition of muscle cell growth and differentiation. The primary pathway involved in this process is the SMAD signaling pathway, which regulates the expression of genes involved in muscle growth .

Recombinant Myostatin

Recombinant myostatin is a form of the protein that is produced using recombinant DNA technology. This involves inserting the gene encoding myostatin into a host organism, such as bacteria or yeast, which then produces the protein. Recombinant myostatin is used in various research applications to study its effects on muscle growth and development .

Clinical Significance

Mutations in the myostatin gene can lead to a condition known as myostatin-related muscle hypertrophy, characterized by increased muscle mass and strength. This condition has been observed in both animals and humans. In animals, such as cattle and dogs, myostatin mutations result in a “double-muscled” phenotype, where the animals have significantly larger muscles than normal .

In humans, myostatin inhibitors are being explored as potential treatments for muscle-wasting conditions, such as muscular dystrophy and sarcopenia (age-related muscle loss). By inhibiting myostatin activity, it may be possible to promote muscle growth and improve muscle function in individuals with these conditions .

Research and Therapeutic Potential

The therapeutic potential of myostatin inhibitors has generated significant interest in the scientific community. Several myostatin inhibitors are currently being developed and tested in clinical trials for their ability to enhance muscle growth and improve muscle function in patients with muscle-wasting diseases .

In conclusion, myostatin is a critical regulator of muscle growth, and recombinant myostatin provides valuable insights into its function and potential therapeutic applications. Ongoing research continues to explore the possibilities of targeting myostatin for the treatment of various muscle-related conditions.

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