Introduction
Myostatin (GDF-8), a member of the transforming growth factor beta (TGFβ) superfamily, is a protein that plays a crucial role in regulating skeletal muscle mass. It acts as a negative regulator, meaning it inhibits muscle growth. Myostatin is found in our bloodstream in an inactive form, bound to other molecules such as its propeptide and follistatin-related gene. The myostatin propeptide is essential because it can bind to myostatin and block its activity. This interaction is significant in living organisms, as a majority (over 70%) of myostatin in the blood is bound to its propeptide, rendering it inactive. Therefore, the myostatin propeptide acts as a natural inhibitor of myostatin's muscle-suppressing effects.
Description
Recombinant Human Myostatin Propeptide is a protein that is artificially produced but structurally similar to the myostatin propeptide found in humans. It has a molecular weight of 27.8 kilodaltons (kDa) and consists of 244 amino acids, which are the building blocks of proteins.
Physical Appearance
The product is a white powder that has been sterilized by filtration and then freeze-dried to remove moisture.
Formulation
The product is freeze-dried without any additional substances.
Solubility
To use the lyophilized (freeze-dried) Myostatin Propeptide, it is recommended to dissolve it in a sterile solution of 20mM hydrochloric acid (HCl) at a concentration of 0.1mg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
To ensure the longevity of the lyophilized protein, it is best to store it at -20°C. Once the protein is reconstituted, it is advisable to divide it into smaller portions to prevent repeated freezing and thawing, which can degrade the protein. The reconstituted protein can be stored at 4°C for a short period.
Purity
The purity of the protein is greater than 95%, as determined by SDS-PAGE, a technique used to separate proteins based on their size.
Biological Activity
The protein demonstrates full biological activity when compared to a standard sample. Its activity is assessed by its ability to inhibit the effects of 50 nanograms per milliliter (ng/ml) of Myostatin on MPC-11 cells, which are muscle cells. The typical effective concentration ranges from 0.13 to 0.2 micrograms per milliliter (µg/ml).
Synonyms
GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.
Amino Acid Sequence
MNENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRR.
