Myc (PMYCSHG) Antibody
Description
Product Specs
Product Science Overview
The Myc (Mouse Antibody) is a monoclonal antibody that specifically recognizes the Myc epitope tag. This tag is widely used in molecular biology and biochemistry for the detection and purification of recombinant proteins. The Myc tag is a short peptide sequence derived from the c-Myc protein, which is a transcription factor involved in cell cycle regulation, apoptosis, and cellular transformation.
Epitope tagging is a technique used to label proteins with a specific peptide sequence (epitope) that can be recognized by an antibody. This allows for the detection, purification, and characterization of the tagged protein. The Myc tag, consisting of the amino acid sequence EQKLISEEDL, is one of the most commonly used epitope tags due to its small size and minimal impact on the protein’s function .
The Myc (Mouse Antibody) is a monoclonal antibody produced by immunizing mice with a synthetic peptide corresponding to residues 410-419 of the human c-Myc protein. This antibody is highly specific for the Myc tag and can be used in various applications, including Western blotting, immunoprecipitation, immunohistochemistry, immunofluorescence, and flow cytometry .
- Western Blotting: The Myc (Mouse Antibody) is used to detect Myc-tagged proteins in cell lysates. It binds to the Myc tag, allowing for the visualization of the protein on a Western blot.
- Immunoprecipitation: This antibody can be used to isolate Myc-tagged proteins from cell extracts. The antibody-protein complex is precipitated, allowing for the purification of the tagged protein.
- Immunohistochemistry: The Myc (Mouse Antibody) is used to detect Myc-tagged proteins in tissue sections. It binds to the Myc tag, allowing for the visualization of the protein within the tissue.
- Immunofluorescence: This antibody can be used to detect Myc-tagged proteins in cells using fluorescently labeled secondary antibodies. This allows for the visualization of the protein within the cell.
- Flow Cytometry: The Myc (Mouse Antibody) can be used to detect Myc-tagged proteins on the surface of cells. This allows for the analysis of protein expression in a population of cells .
