Myc-HRP Antibody

The Myc (Mouse Antibody Peroxidase Conjugated) is a monoclonal antibody that is widely used in various biochemical and molecular biology applications. This antibody is specifically designed to recognize and bind to the Myc epitope tag, which is often fused to proteins of interest to facilitate their detection and purification.

The Myc epitope tag is a short peptide sequence derived from the c-Myc protein, a proto-oncogene that plays a crucial role in cell cycle regulation, apoptosis, and cellular transformation. The Myc tag sequence is typically EQKLISEEDL, and it is commonly used in recombinant DNA technology to tag proteins for various experimental purposes .

The Myc antibody is produced by immunizing mice with a synthetic peptide corresponding to the Myc tag sequence. The immune response generates B cells that produce antibodies specific to the Myc tag. These B cells are then fused with myeloma cells to create hybridoma cells, which can be cultured to produce large quantities of the monoclonal antibody .

To enhance the detection capabilities of the Myc antibody, it is often conjugated to an enzyme such as horseradish peroxidase (HRP). The peroxidase enzyme catalyzes the oxidation of substrates in the presence of hydrogen peroxide, producing a detectable signal, usually in the form of a color change. This conjugation allows for the sensitive detection of Myc-tagged proteins in various assays, including Western blotting, immunohistochemistry, and ELISA .

The Myc (Mouse Antibody Peroxidase Conjugated) is used in a variety of applications:

• Western Blotting: To detect Myc-tagged proteins separated by SDS-PAGE.
• Immunoprecipitation: To isolate Myc-tagged proteins from cell lysates.
• Immunohistochemistry: To visualize Myc-tagged proteins in tissue sections.
• ELISA: To quantify Myc-tagged proteins in samples.