c-myc Antibody

The c-Myc protein is a transcription factor encoded by the c-Myc gene located on human chromosome 8q24 . It plays a crucial role in various cellular processes, including proliferation, differentiation, apoptosis, and cell cycle progression . The c-Myc protein is commonly activated in a variety of tumor cells, making it a significant focus in cancer research .

c-Myc is a 62 kDa protein that functions as a transcription factor by binding to specific DNA sequences to regulate the expression of target genes . It is involved in the regulation of several cellular processes:

• Cell Proliferation: c-Myc promotes cell growth and division by regulating genes involved in the cell cycle .
• Differentiation: It influences the differentiation of various cell types .
• Apoptosis: c-Myc can induce programmed cell death under certain conditions .
• Cell Cycle Progression: It plays a role in the transition between different phases of the cell cycle .

c-Myc is constitutively and aberrantly expressed in over 70% of human cancers . Its overexpression is associated with tumorigenesis, making it a potential target for cancer therapy . Direct inhibition of c-Myc has been shown to trigger rapid tumor regression in mice with only mild and fully reversible side effects .

Mouse anti-human c-Myc antibodies are monoclonal antibodies developed to target the c-Myc protein in human samples . These antibodies are widely used in various scientific applications, including:

• Western Blot: To detect c-Myc protein levels in different samples .
• Immunohistochemistry (IHC): To visualize c-Myc expression in tissue sections .
• Immunocytochemistry (ICC): To study c-Myc localization in cultured cells .
• Immunoprecipitation (IP): To isolate c-Myc protein complexes from cell lysates .
• Enzyme-Linked Immunosorbent Assay (ELISA): To quantify c-Myc protein levels in various samples .

Mouse anti-human c-Myc antibodies are typically generated by immunizing mice with a synthetic peptide corresponding to residues 410-419 of the human c-Myc protein . These antibodies are highly specific and can bind to c-Myc in its denatured form, relying on a linear and continuous epitope .

The use of mouse anti-human c-Myc antibodies has been instrumental in advancing our understanding of c-Myc’s role in cancer and other diseases. These antibodies have been validated in numerous experiments and publications, making them reliable tools for scientific research .