Prolactin Mouse, His
AV290867, Gha1, Prl1a1, Growth hormone a1, Mammotropin, Luterotropic hormone, Lutetropin, PRL.
Description
Prolactin Mouse produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 222 amino acids (30-228a.a.) and having a molecular mass of 25 kDa.
Prolactin Mouse protein is fused to a 23 amino acid His tag at N-terminus and is purified by standard chromatography.
Product Specs
AV290867, Gha1, Prl1a1, Growth hormone a1, Mammotropin, Luterotropic hormone, Lutetropin, PRL.
MGSSHHHHHH SSGLVPRGSH MGSQPLPICS AGDCQTSLRE LFDRVVILSH YIHTLYTDMF IEFDKQYVQD REFMVKVIND CPTSSLATPE DKEQALKVPP EVLLNLILSL VQSSSDPLFQ LITGVGGIQE APEYILSRAK EIEEQNKQLL EGVEKIISQA YPEAKGNGIY VWSQLPSLQ GVDEESKILS LRNTIRCLRR DSHKVDNFLK VLRCQIAHQN NC.
Product Science Overview
Prolactin is involved in several critical physiological processes:
- Water and Electrolyte Balance: PRL helps regulate the balance of water and electrolytes in the body.
- Reproduction: It plays a significant role in reproductive functions, including lactation.
- Growth and Development: PRL contributes to growth and developmental processes.
- Metabolism: It influences metabolic activities.
- Immuno-modulation: PRL has a role in modulating the immune system.
- Behavior: It affects various behavioral aspects.
Recombinant mouse prolactin with a His tag is a laboratory-produced version of the natural hormone. It is expressed in baculovirus-insect cells and includes a polyhistidine tag at the C-terminus for purification purposes.
Key Characteristics:
- Expression System: Baculovirus-insect cells.
- Molecular Weight: The recombinant mouse PRL consists of 207 amino acids and has a calculated molecular mass of 23.8 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions .
- Purity: ≥ 92% as determined by SDS-PAGE and ≥ 90% as determined by SEC-HPLC .
- Endotoxin Level: Less than 1.0 EU per μg of the protein as determined by the LAL method .
- Biological Activity: Measured by its ability to induce PRL pathway activation in a Luciferase receptor Assay System. The ED50 for this effect is typically 50-500 ng/mL .
Formulation and Storage:
- Buffer: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol .
- Stability: Samples are stable for up to twelve months from the date of receipt at -20°C to -80°C. It is recommended to store the protein under sterile conditions and avoid repeated freeze-thaw cycles .
Recombinant mouse prolactin with a His tag is a valuable tool for research, allowing scientists to study the hormone’s functions and interactions in various biological processes.
