Prolactin Human, His
Prolactin Human Recombinant, His Tag
Cat. No.
BT30242
Source
Escherichia Coli.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Prolactin-His Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids fragment (29-227) and having a molecular mass of 23 kDa with an amino-terminal hexahistidine tag.
The Prolactin is purified by proprietary chromatographic techniques.
The Prolactin is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Prolactin, a neuroendocrine hormone primarily produced by the pituitary gland, is also synthesized by other cells like those in the placenta, brain, and uterus. While its main function is to stimulate and maintain milk production, it is also involved in breast cancer development, reproductive function regulation, and immunoregulation.
Description
Recombinant Human Prolactin-His, expressed in E.Coli, is a single, non-glycosylated polypeptide chain. It consists of 199 amino acids (fragment 29-227), has a molecular weight of 23 kDa, and includes an amino-terminal hexahistidine tag. The purification process for Prolactin utilizes proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
Prolactin His is provided in a solution containing 1x PBS and 50% glycerol.
Stability
For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For longer storage periods, freeze at -20°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 95.0% as determined by (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Synonyms
Mammotropin, Luteotropic hormone, Luteotropin, PRL.
Source
Escherichia Coli.
Product Science Overview
Recombinant Human Prolactin (His Tag)
Recombinant Human Prolactin (His Tag) is a form of prolactin that has been genetically engineered to include a polyhistidine tag (His tag) at the C-terminus. This tag facilitates the purification and detection of the protein. The recombinant protein is typically expressed in baculovirus-infected insect cells .
Key Characteristics:
- Protein Construction: The DNA sequence encoding human PRL (Leu29-Cys227) is expressed with a polyhistidine tag at the C-terminus .
- Molecular Mass: The recombinant human PRL consists of 210 amino acids and has a predicted molecular mass of 24.3 kDa .
- Purity: The protein has a purity of over 90% as determined by SDS-PAGE .
- Endotoxin Level: The endotoxin level is less than 1.0 EU per μg protein as determined by the LAL method .
Biological Activity: Recombinant Human Prolactin (His Tag) is measured by its ability to induce PRL pathway activation in a Luciferase receptor Assay System. The effective dose (ED50) for this effect is typically 5-50 ng/mL .
Formulation and Storage:
- Formulation: The protein is lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycine, pH 8.0. Additional protectants such as trehalose, mannitol, and Tween80 may be added before lyophilization .
- Storage: Samples are stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles .
Applications: Recombinant Human Prolactin (His Tag) is used in various research applications, including:
- Studying PRL receptor (PRLR) activation: Understanding the signaling pathways and physiological effects mediated by PRL .
- Investigating prolactin’s role in the central nervous system (CNS): PRL exerts different actions through its receptors found in neurons and glial cells of the brain .
- Exploring prolactin’s involvement in autoimmune diseases: Research has shown a link between prolactin and autoimmunity .
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