MORF4L2 Human
Mortality factor 4-like protein 2, MORF-related gene X protein, Protein MSL3-2, Transcription factor-like protein MRGX, MORF4L2, KIAA0026, MRGX, MORFL2.
Greater than 85.0% as determined by SDS-PAGE.
Description
MORF4L2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-288 a.a.) and having a molecular mass of 34.4kDa (Molecular weight on SDS-PAGE will appear higher).
MORF4L2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Mortality factor 4-like protein 2, MORF-related gene X protein, Protein MSL3-2, Transcription factor-like protein MRGX, MORF4L2, KIAA0026, MRGX, MORFL2.
MGSSHHHHHH SSGLVPRGSH MSSRKQGSQP RGQQSAEEEN FKKPTRSNMQ RSKMRGASSG KKTAGPQQKN LEPALPGRWG GRSAENPPSG SVRKTRKNKQ KTPGNGDGGS TSEAPQPPRK KRARADPTVE SEEAFKNRME VKVKIPEELK PWLVEDWDLV TRQKQLFQLP AKKNVDAILE EYANCKKSQG NVDNKEYAVN EVVAGIKEYF NVMLGTQLLY KFERPQYAEI LLAHPDAPMS QVYGAPHLLR LFVRIGAMLA YTPLDEKSLA LLLGYLHDFL KYLAKNSASL FTASDYKVAS AEYHRKAL.
Product Science Overview
Mortality Factor 4 Like 2, also known as MORF4L2, is a protein-coding gene that plays a significant role in various cellular processes. This protein is a component of the NuA4 histone acetyltransferase complex, which is involved in transcriptional activation of select genes by acetylation of nucleosomal histone H4 and H2A . This modification can alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins that positively regulate transcription .
MORF4L2 is predicted to be involved in heterochromatin assembly and histone modification . It acts upstream of or within the positive regulation of striated muscle cell differentiation and transcription by RNA polymerase II . The protein is located in the nucleolus, nucleoplasm, and plasma membrane . It is also a component of the MSIN3A complex, which represses transcription by deacetylation of nucleosomal histones .
Recombinant Human Mortality Factor 4 Like 2 can be prepared using various methods. One common method involves expressing the protein in a suitable host system, such as Escherichia coli, followed by purification using affinity chromatography . The purified protein is then formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, and 1mM DTT .
MORF4L2 is a part of the NuA4 histone acetyltransferase complex, which is involved in the acetylation of nucleosomal histone H4 and H2A . This acetylation process is crucial for transcriptional activation and can influence various cellular processes, including growth induction, tumor suppressor-mediated growth arrest, replicative senescence, apoptosis, and DNA repair . The NuA4 complex’s ATPase and helicase activities are contributed by the association of RUVBL1 and RUVBL2 with EP400 .
