MMP9 Mouse
HEK293 Cells.
AW743869, Matrix metalloproteinase-9, 92 kDa type IV collagenase, Gelatinase B, GELB, Mmp9, MANDP2, B/MMP, B/MMP9, Clg4, Clg4b, Gel B, MMP-9, pro-MMP-9, 92 kDa gelatinase.
Greater than 90.0% as determined by SDS-PAGE.
Description
MMP9 Mouse Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (20-730 a.a) containing a total of 717 amino acids, having a molecular mass of 79.3kDa.
MMP9 is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
Product Specs
AW743869, Matrix metalloproteinase-9, 92 kDa type IV collagenase, Gelatinase B, GELB, Mmp9, MANDP2, B/MMP, B/MMP9, Clg4, Clg4b, Gel B, MMP-9, pro-MMP-9, 92 kDa gelatinase.
HEK293 Cells.
APYQRQPTFV VFPKDLKTSN LTDTQLAEAY LYRYGYTRAA QMMGEKQSLR PALLMLQKQL SLPQTGELDS QTLKAIRTPR CGVPDVGRFQ TFKGLKWDHH NITYWIQNYS EDLPRDMIDD AFARAFAVWG EVAPLTFTRV YGPEADIVIQ FGVAEHGDGY PFDGKDGLLA HAFPPGAGVQ GDAHFDDDEL WSLGKGVVIP TYYGNSNGAP CHFPFTFEGR SYSACTTDGR NDGTPWCSTT ADYDKDGKFG FCPSERLYTE HGNGEGKPCV FPFIFEGRSY SACTTKGRSD GYRWCATTAN YDQDKLYGFC PTRVDATVVG GNSAGELCVF PFVFLGKQYS SCTSDGRRDG RLWCATTSNF DTDKKWGFCP DQGYSLFLVA AHEFGHALGL DHSSVPEALM YPLYSYLEGF PLNKDDIDGI QYLYGRGSKP DPRPPATTTT EPQPTAPPTM CPTIPPTAYP TVGPTVGPTG APSPGPTSSP SPGPTGAPSP GPTAPPTAGS SEASTESLSP ADNPCNVDVF DAIAEIQGAL HFFKDGWYWK FLNHRGSPLQ GPFLTARTWP ALPATLDSAF EDPQTKRVFF FSGRQMWVYT GKTVLGPRSL DKLGLGPEVT HVSGLLPRRL GKALLFSKGR VWRFDLKSQK VDPQSVIRVD KEFSGVPWNS HDIFQYQDKA YFCHGKFFWR VSFQNEVNKV DHEVNQVDDV GYVTYDLLQC PHHHHHH.
Product Science Overview
MMP-9 is synthesized as an inactive proenzyme (proMMP-9) and requires activation to become functionally active. The activation process involves the cleavage of the propeptide domain, which exposes the active site of the enzyme. The recombinant mouse MMP-9 protein is typically produced in a pro form and requires activation by agents such as p-aminophenylmercuric acetate (APMA) to achieve its active state .
MMP-9 is involved in various physiological and pathological processes, including:
- Tissue Remodeling: MMP-9 plays a significant role in tissue remodeling by degrading ECM components, which is essential for processes such as wound healing and embryonic development.
- Inflammatory Responses: MMP-9 is produced by various cell types, including monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, and endothelial cells. It is involved in the regulation of inflammatory responses by modulating the activity of cytokines and chemokines .
- Tumor Growth and Metastasis: MMP-9 is implicated in cancer progression by promoting tumor growth, angiogenesis, and metastasis. It facilitates the invasion of cancer cells by degrading the basement membrane and ECM components .
Recombinant MMP-9 is widely used in research to study its role in various biological processes and diseases. It is also utilized in the development of inhibitors and therapeutic agents targeting MMP-9 activity. The ability to produce recombinant MMP-9 with high purity and activity has opened new avenues for understanding its function and developing potential treatments for MMP-9-related diseases .
