Midkine Mouse
Midkine Mouse Recombinant
Cat. No.
BT9690
Source
Escherichia Coli.
Synonyms
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by HPLC and SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Midkine Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.3kDa.
The Midkine Mouse is purified by proprietary chromatographic techniques.
The Midkine Mouse is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Midkine (MK) belongs to a family of heparin-binding growth factors and is encoded by a gene responsive to retinoic acid. Composed of 121 amino acids, it features 10 cysteine residues that are conserved and likely form disulfide bonds. During embryonic development, MK exhibits a distinct expression pattern suggestive of roles in neurogenesis, cell migration, organ development, and interactions between mesoderm and epithelium. While its presence is largely downregulated in adult humans, MK expression resurfaces in various cancers, where it can function as both a growth factor promoting transformation and a contributor to angiogenesis. Notably, MK exhibits chemotactic activity on human neutrophils, prompting their migration, and can trigger calcium mobilization within these cells. Moreover, it acts as a potent stimulator of histamine release from rat peritoneal mast cells in a rapid and dose-dependent fashion. Furthermore, MK demonstrates the ability to stimulate the production of collagen and glycosaminoglycans.
Description
Recombinant Mouse Midkine, produced in E. coli, is a single-chain polypeptide lacking glycosylation. It consists of 120 amino acids and has a molecular weight of 13.3 kDa. The purification process for this recombinant protein involves proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile-filtered.
Formulation
Lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute lyophilized Mouse Midkine, it is recommended to dissolve it in sterile 18M-cm H₂O at a concentration of at least 100 µg/ml. The resulting solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Mouse Midkine, though stable at room temperature for up to 3 weeks, should be stored in a dry environment below -18°C for long-term preservation. Once reconstituted, it can be kept at 4°C for 2-7 days. For extended storage, freezing at -18°C is recommended. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0%, as determined by high-performance liquid chromatography (HPLC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The biological activity is comparable to the standard, as assessed by its chemotactic effect on human neutrophils. Within a concentration range of 10-100 ng/ml, it exhibits a specific activity of 10,000-100,000 IU/mg.
Synonyms
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Source
Escherichia Coli.
Amino Acid Sequence
VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
Product Science Overview
Discovery and Structure
Midkine was originally identified as a cytokine highly expressed during mid-gestation in many organs of the mouse, particularly the kidneys, heart, and brain . It is a highly basic, non-glycosylated polypeptide consisting of two domains stabilized by five intrachain disulfide bonds . The protein has a molecular weight of approximately 13 kDa .
Biological Functions
Midkine is involved in a variety of biological functions, including:
- Cell Proliferation and Migration: MK promotes cell proliferation, migration, and differentiation through interactions with multiple cell surface receptors .
- Neuroprotection and Neuroregeneration: MK is upregulated in the adult central nervous system (CNS) after various types of experimental injury. It has neuroprotective and neuroregenerative properties, making it a promising candidate for therapeutic applications in brain injuries .
- Developmental Roles: During embryonic and fetal development, MK is highly expressed and plays a significant role in the structural and functional development of the brain .
Recombinant Mouse Midkine
Recombinant mouse midkine is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications . It is often used to study its effects on neurite outgrowth, cell proliferation, and other biological processes .
Therapeutic Potential
The potential for MK as a therapeutic agent is being explored in various fields, including:
- Perinatal Brain Injury: MK has shown promise as a neurotrophic therapy for perinatal brain injury, where it can ameliorate the effects of brain damage caused by hypoxia and inflammation .
- Cancer: MK expression is induced in many forms of cancer, where it mediates hypoxic or inflammatory-driven cell response pathways . Research is ongoing to explore its role in cancer therapy.
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