Midkine Human
Midkine Human Recombinant
Cat. No.
BT9514
Source
Escherichia Coli.
Synonyms
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Midkine Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 123 amino acids and having a molecular mass of 13.4kDa.
The Midkine is purified by proprietary chromatographic techniques.
The Midkine is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Midkine (MK) is a growth factor induced by retinoic acid. It consists of 121 amino acids, including 10 cysteine residues that form disulfide bonds. MK is primarily expressed during embryonic development and plays a role in various processes like neurogenesis, cell migration, organ development, and interactions between mesoderm and epithelium. In adults, MK expression decreases but can be reactivated in certain cancers, where it promotes tumor growth and blood vessel formation. MK attracts neutrophils (a type of white blood cell) and triggers their activation by increasing intracellular calcium levels. It also stimulates the release of histamine from mast cells in a dose-dependent manner. Additionally, MK promotes the production of collagen and glycosaminoglycans, which are essential components of the extracellular matrix.
Description
Recombinant Human Midkine, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 123 amino acids. It has a molecular weight of 13.4 kDa. This Midkine product is purified using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized powder, sterile-filtered.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, with a pH of 7.4.
Solubility
To reconstitute the lyophilized Midkine, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O at a concentration of at least 100 µg/ml. The resulting solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Midkine remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, Midkine should be stored at 4°C for a period of 2-7 days. For extended storage, adding a carrier protein like HSA or BSA at a concentration of 0.1% is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 95%, as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis. (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity is assessed by measuring its ability to attract human neutrophils. This is done within a concentration range of 0.1-10 ng/ml, which corresponds to a specific activity of 100,000-10,000,000 IU/mg.
Synonyms
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Source
Escherichia Coli.
Amino Acid Sequence
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD.
Product Science Overview
Structure and Characteristics
Biological Functions
Midkine plays a crucial role in various biological processes, including:
- Cell Proliferation and Migration: It promotes the proliferation and migration of cells, which is essential for tissue repair and regeneration .
- Angiogenesis: Midkine stimulates the formation of new blood vessels, a process vital for wound healing and tumor growth .
- Neurite Extension and Neuronal Survival: It enhances neurite outgrowth and supports the survival of neurons, making it significant in neural development and repair .
- Oncogenesis and Inflammation: Midkine is strongly induced during oncogenesis and inflammation, indicating its role in cancer progression and inflammatory responses .
Developmental Importance
Recombinant Human Midkine
Recombinant human midkine is produced using E. coli expression systems. The recombinant protein is typically purified to high levels of purity (>97%) and is used in various research applications . It is available in carrier-free formulations, which are suitable for applications where the presence of carrier proteins like BSA could interfere .
Applications in Research
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
