Midkine Human, His

Midkine Human Recombinant, His Tag
Cat. No.
BT9640
Source
Escherichia Coli.
Synonyms
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Midkine Human Recombinant is manufactured with N-terminal fusion of His Tag, having a molecular mass of 14.6 kDa protein and containing 121 amino acid residues of the Midkine human and 10 additional amino acid residues – His Tag (underlined).

Product Specs

Introduction
Midkine (MK) is a heparin-binding growth factor involved in various biological processes, including cell growth, differentiation, and migration. It plays a crucial role in embryogenesis and is implicated in several cancers and inflammatory conditions.
Description
Recombinant human Midkine protein, expressed in a suitable host system, with an N-terminal His-tag for purification. The protein has a molecular weight of approximately 14.6 kDa and consists of 121 amino acids of the mature human Midkine sequence and a 10 amino acid His-tag.
Physical Appearance
White lyophilized powder.
Formulation
The protein is lyophilized from a solution containing 0.5 mg/ml Midkine in 0.05 M phosphate buffer (pH 7.2) and 0.1 M NaCl.
Solubility
To reconstitute the lyophilized protein, add 0.2 ml of phosphate-buffered saline (PBS) at pH 7.2 to the vial and allow the pellet to dissolve completely.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the protein and store at 4°C for short-term use (up to 2 weeks). Avoid repeated freeze-thaw cycles.
Purity
The purity of the recombinant Midkine protein is greater than 95% as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Synonyms
NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D.

Product Science Overview

Structure and Expression

Recombinant human Midkine protein, fused to a His-tag at the N-terminus, is expressed in Escherichia coli and purified using conventional chromatography . The protein consists of amino acids 21-143 of human Midkine and has a theoretical molecular weight of approximately 15.7 kDa . The His-tag facilitates purification and detection of the protein.

Biological Functions

Midkine is pleiotropic, capable of exerting various biological activities such as:

  • Cell proliferation
  • Cell migration
  • Angiogenesis
  • Fibrinolysis

It binds to cell-surface proteoglycan receptors via their chondroitin sulfate (CS) groups, regulating processes like inflammatory response, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation, and cell migration .

Applications

Recombinant human Midkine protein is used in research to study its role in various biological processes and its potential therapeutic applications. It is particularly valuable in studies related to:

  • Nervous system development
  • Cancer research
  • Tissue regeneration
Storage and Handling

The protein is typically stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain its stability and activity .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Midkine Human
Midkine Human Recombinant
Cat. No.
BT9514
Source
Escherichia Coli.
Midkine Mouse
Midkine Mouse Recombinant
Cat. No.
BT9690
Source
Escherichia Coli.
Midkine Rat
Midkine Rat Recombinant
Cat. No.
BT9781
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.