MFAP4 Human, Sf9

Microfibrillar-associated Protein 4 Human Recombinant, Sf9
Cat. No.
BT12352
Source
Sf9, Baculovirus cells.
Synonyms
Microfibrillar Associated Protein 4, Microfibril-Associated Glycoprotein 4, Microfibril-associated glycoprotein 4.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MFAP4 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 243 amino acids (22-255a.a) and having a molecular mass of 27.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). 
MFAP4 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Microfibrillar-associated protein 4 (MFAP4) is a member of the Fibrinogen protein family and contains one fibrinogen C-terminal domain. This protein shares similarities with a bovine microfibril-associated protein and exhibits binding affinity for both collagen and carbohydrates. MFAP4 is thought to be an extracellular matrix protein with a role in cell adhesion or intercellular interactions. Notably, MFAP4 deletion has been observed in a significant majority (30 out of 31) of Smith-Magenis syndrome (SMS) patients.
Description
MFAP4 Human Recombinant, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 243 amino acids (22-255a.a). It possesses a molecular mass of 27.5kDa, though on SDS-PAGE analysis, the apparent molecular size might appear between 28-40kDa. This discrepancy can be attributed to glycosylation. The protein is engineered with a 6 amino acid His-tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has undergone sterile filtration.
Formulation
The MFAP4 protein solution is provided at a concentration of 1mg/ml. The formulation buffer consists of Phosphate Buffered Saline at a pH of 7.4 and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of MFAP4 Human Recombinant is determined to be greater than 90.0% based on SDS-PAGE analysis.
Synonyms
Microfibrillar Associated Protein 4, Microfibril-Associated Glycoprotein 4, Microfibril-associated glycoprotein 4.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADLVSGIRGD ALERFCLQQP LDCDDIYAQG YQSDGVYLIY PSGPSVPVPV FCDMTTEGGK WTVFQKRFNG SVSFFRGWND YKLGFGRADG EYWLGLQNMH LLTLKQKYEL RVDLEDFENN TAYAKYADFS ISPNAVSAEE DGYTLFVAGF EDGGAGDSLS YHSGQKFSTF DRDQDLFVQN CAALSSGAFW FRSCHFANLN GFYLGGSHLS YANGINWAQW KGFYYSLKRT EMKIRRAHHH HHH.

Product Science Overview

Gene and Protein Structure

The MFAP4 gene encodes a protein with similarity to a bovine microfibril-associated protein . The protein is thought to contribute to the assembly and maintenance of elastic fibers . The gene is located within the Smith-Magenis syndrome region, and two transcript variants encoding different isoforms have been identified .

Expression and Localization

MFAP4 is primarily located in the extracellular matrix (ECM) and is highly expressed in tissues rich in elastic fibers, such as the heart, lung, and intestine . Immunohistochemical studies have shown that MFAP4 is mainly found within blood vessels in all tissues investigated .

Clinical Significance

MFAP4 has been evaluated as a marker for various cardiovascular conditions. Studies have shown that serum MFAP4 levels vary among patients with different cardiovascular diseases . For instance, lower serum MFAP4 levels have been observed in patients with stable atherosclerotic disease compared to those with ST elevation myocardial infarction (STEMI) and non-STEMI .

Recombinant Production

The human recombinant MFAP4 protein produced in Sf9 cells is used for various research purposes. Sf9 cells, derived from the fall armyworm Spodoptera frugiperda, are commonly used in recombinant protein production due to their high expression levels and ability to perform post-translational modifications .

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