MFAP4 Human

Microfibrillar-associated Protein 4 Human Recombinant
Cat. No.
BT12258
Source
E.coli.
Synonyms
Microfibrillar-Associated Protein 4, Microfibril-Associated Glycoprotein 4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MFAP4 Human Recombinant produced in E. coli is a single polypeptide chain containing 259 amino acids (22-255) and having a molecular mass of 29.2 kDa.
MFAP4 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Microfibrillar-associated protein 4 (MFAP4) is a protein that is part of the Fibrinogen family. It contains a fibrinogen C-terminal domain and shares similarities with a microfibril-associated protein found in cows. MFAP4 can bind to both collagen and carbohydrates. It is thought to be a protein found outside of cells, in the extracellular matrix, where it plays a role in cell adhesion or interactions between cells. Studies have shown that a deletion of the MFAP4 gene is present in a significant majority of patients with Smith-Magenis syndrome (SMS).
Description
This product is a recombinant human MFAP4 protein produced in E. coli bacteria. It consists of a single polypeptide chain with 259 amino acids (specifically, amino acids 22 to 255 of the full protein sequence) and has a molecular weight of 29.2 kDa. The MFAP4 protein is attached to a 25 amino acid His-tag at its N-terminus. It has been purified using specialized chromatographic methods to ensure its purity.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
This MFAP4 solution has a concentration of 1mg/ml. It is supplied in a buffer solution containing 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at a refrigerated temperature of 4°C. For longer storage periods, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of this product is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Microfibrillar-Associated Protein 4, Microfibril-Associated Glycoprotein 4.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVSGIR GDALERFCLQ QPLDCDDIYA QGYQSDGVYL IYPSGPSVPV PVFCDMTTEG GKWTVFQKRF NGSVSFFRGW NDYKLGFGRA DGEYWLGLQN MHLLTLKQKY ELRVDLEDFE NNTAYAKYAD FSISPNAVSA EEDGYTLFVA GFEDGGAGDS LSYHSGQKFS TFDRDQDLFV QNCAALSSGA FWFRSCHFAN LNGFYLGGSH LSYANGINWA QWKGFYYSLK RTEMKIRRA

Product Science Overview

Structure and Function

MFAP4 belongs to the fibrinogen-related domain (FReD) family, which includes proteins involved in tissue homeostasis and innate immunity . The protein has binding specificities for both collagen and carbohydrates, suggesting its involvement in cell adhesion and intercellular interactions . MFAP4 primarily assembles into trimeric and hexameric structures of homodimers .

Molecular Interactions

MFAP4 specifically binds to tropoelastin, fibrillin-1, and fibrillin-2, as well as the elastin cross-linking amino acid desmosine . These interactions are crucial for the proper organization and assembly of elastic fibers. MFAP4 has been shown to co-localize with fibrillin-1-positive fibers in vivo, indicating its role in elastic fiber formation .

Biological Significance

The protein is thought to contribute to the assembly and maintenance of elastic fibers, which are essential for the elasticity and resilience of various tissues, including skin, lungs, and blood vessels . MFAP4’s involvement in elastic fiber formation makes it a key player in maintaining the structural integrity of these tissues.

Clinical Relevance

Mutations or dysregulation of the MFAP4 gene have been associated with several disorders, including Smith-Magenis syndrome and Robinow syndrome . Understanding the role of MFAP4 in these conditions could provide insights into potential therapeutic targets.

Human Recombinant MFAP4

Human recombinant MFAP4 is produced using recombinant DNA technology, which involves inserting the MFAP4 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the large-scale production of the protein for research and therapeutic purposes.

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