MESDC2 Human

Mesoderm Development Candidate 2 (MESDC2), also known as Mesoderm Development LRP Chaperone, is a protein encoded by the MESDC2 gene in Homo sapiens. This protein plays a crucial role in embryogenesis, particularly in the formation of the primitive streak and mesoderm. MESDC2 is involved in the proper folding and cell surface expression of Wnt co-receptors LRP5 and LRP6, which are essential for the Wnt signaling pathway .

The MESDC2 gene is predominantly expressed in the thyroid, placenta, and adrenal glands. It is also found in other tissues but at lower levels. The protein is localized in the endoplasmic reticulum, where it binds to LRP5 and LRP6, ensuring their proper folding and preventing the formation of aggregates .

MESDC2 has several critical functions:

1. Chaperone Activity: MESDC2 assists in the folding of beta-propeller/EGF modules within the low-density lipoprotein receptor (LDLR) family.
2. Wnt Pathway Modulation: It guides LRP5 and LRP6 to the plasma membrane, modulating the Wnt signaling pathway.
3. Embryonic Development: MESDC2 is vital for specifying embryonic polarity and mesoderm induction.
4. Neuromuscular Junction Formation: It promotes cell-surface expression of LRP4, which is essential for neuromuscular junction formation .

MESDC2 Human Recombinant is produced in E. coli as a single, non-glycosylated polypeptide chain containing 222 amino acids. The recombinant protein is fused with a 21 amino acid His tag at the N-terminus and has a molecular mass of approximately 24.9 kDa. The protein is purified using proprietary chromatographic techniques to ensure high purity .

The MESDC2 solution is formulated with 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl, and 1mM DTT. It is a sterile filtered colorless solution. The protein is stable when stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is recommended to add a carrier protein (0.1% HSA or BSA) for extended storage to avoid multiple freeze-thaw cycles .