MESDC1 Human
Description
MESDC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
MESDC1 is a member of the MESD family and consists of 362 amino acids . It functions primarily as a chaperone for low-density lipoprotein receptor (LDLR) proteins within the endoplasmic reticulum (ER). This chaperone activity is essential for the proper folding and functioning of these receptor proteins.
One of the significant roles of MESDC1 is its association with the Wnt signaling pathway. It specifically interacts with the coreceptors LRP5 and LRP6, which are crucial components of this pathway . The Wnt signaling pathway is vital for various developmental processes, including cell proliferation, differentiation, and migration.
Recombinant MESDC1 can be prepared using various biotechnological methods. Typically, the MESDC1 gene is cloned into an expression vector, which is then introduced into a suitable host cell, such as E. coli or mammalian cells. The host cells express the MESDC1 protein, which can be purified using techniques like affinity chromatography. The purified protein is then formulated in a buffer solution containing Tris-HCl, NaCl, glycerol, and DTT to maintain its stability .
MESDC1 undergoes several post-translational modifications that are essential for its function. These modifications include glycosylation and disulfide bond formation, which help in maintaining the protein’s structure and stability. The interaction of MESDC1 with LDLR proteins and Wnt signaling coreceptors involves specific binding sites and conformational changes that are critical for its chaperone activity.
