MDC Rat

Macrophage-Derived Chemokine (CCL22) Rat Recombinant
Cat. No.
BT18889
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 22, Small-inducible cytokine A22, Macrophage-derived chemokine, MDC(1-69), Stimulated T-cell chemotactic protein 1, CC chemokine STCP-1, CCL22, MDC, SCYA22, ABCD-1, DC/B-CK, MGC34554, A-152E5.1, CC chemokine ABCD-1, Activated B and dendritic cell-derived, DCBCK.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CCL22 Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 7.9kDa.
The Rat CCL22 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Macrophage-derived chemokine (MDC), also known as CCL22, is a small cytokine belonging to the CC chemokine family. The gene encoding CCL22 is located on chromosome 16 among a cluster of other CC chemokine genes. MDC exhibits chemotactic activity towards various immune cells, including natural killer (NK) cells, chronically activated T lymphocytes, monocytes, and dendritic cells. While it demonstrates mild activity towards primary activated T lymphocytes, it does not attract neutrophils, eosinophils, or resting T lymphocytes. MDC is believed to play a role in directing activated T lymphocytes to sites of inflammation and influencing other aspects of their physiology. It exerts its effects by binding to the cell surface chemokine receptor CCR4. CCL22 is predominantly expressed in macrophages, monocyte-derived dendritic cells, and the thymus. It is also found in lymph nodes, the appendix, activated monocytes, and resting and activated macrophages. Lower levels of CCL22 expression are detected in the lungs and spleen, with very weak expression observed in the small intestine. Within lymph nodes, CCL22 expression is localized to a mature subset of Langerhans cells characterized by the surface markers CD1a+ and CD83+. Elevated expression of CCL22 has been observed in various inflammatory skin conditions, including atopic dermatitis, allergic contact dermatitis, and psoriasis, affecting both the epidermis and dermis. Furthermore, CCL22 is thought to play a role in inhibiting the progression of lung cancer. Notably, a significant increase in CCL22 expression has been linked to gastric cancer.
Description
Recombinant Rat CCL22, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 68 amino acids. It has a molecular weight of 7.9 kDa. The purification process for Rat CCL22 involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a 0.2 μm filtered solution in 1xPBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized MDC in sterile 18 MΩ-cm H₂O at a concentration of not less than 100 μg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized MDC, though stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, MDC should be stored at 4°C for 2-7 days and below -18°C for future use. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Greater than 97.0% as determined by (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The biological activity of CCL22 is determined by its ability to induce chemotaxis in human activated lymphocytes. This is measured using a concentration range of 10.0-100.0 ng/ml.
Synonyms
C-C motif chemokine 22, Small-inducible cytokine A22, Macrophage-derived chemokine, MDC(1-69), Stimulated T-cell chemotactic protein 1, CC chemokine STCP-1, CCL22, MDC, SCYA22, ABCD-1, DC/B-CK, MGC34554, A-152E5.1, CC chemokine ABCD-1, Activated B and dendritic cell-derived, DCBCK.
Source
Escherichia Coli.
Amino Acid Sequence
GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA.

Product Science Overview

Introduction

Macrophage-Derived Chemokine (CCL22), also known as MDC, is a member of the CC chemokine family. Chemokines are small cytokines or signaling proteins secreted by cells, and they play a crucial role in immune responses by directing the migration of immune cells to sites of inflammation, infection, and trauma. CCL22 is encoded by the CCL22 gene and is highly conserved across species, including rats and humans.

Gene and Protein Structure

The CCL22 gene is located on chromosome 16 in humans and encodes a protein that shares 37% identity with CCL17 at the amino acid level . The recombinant rat CCL22 is a single, non-glycosylated polypeptide chain consisting of 68 amino acids with a molecular weight of approximately 7.9 kDa . The amino acid sequence of rat CCL22 is as follows: GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA .

Expression and Secretion

CCL22 is predominantly expressed in macrophages, monocyte-derived dendritic cells, and the thymus . It is also detected in tissues such as the lymph nodes and appendix . Upon secretion from monocyte-derived dendritic cells, CCL22 can be proteolytically cleaved into three forms: MDC (3-69), MDC (5-69), and MDC (7-69) .

Biological Activity

CCL22 exerts its effects by binding to the CCR4 receptor . It acts as a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer (NK) cells, but not for neutrophils, eosinophils, or resting T-lymphocytes . Additionally, CCL22 has been shown to have HIV suppressive activity . The biological activity of recombinant rat CCL22 is confirmed through chemotaxis bioassays using human T-lymphocytes, with effective concentrations ranging from 10 to 100 ng/ml .

Applications and Usage

Recombinant rat CCL22 is widely used in research to study immune responses, cell signaling, and chemokine-receptor interactions. It is available in lyophilized form and can be reconstituted in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml . The protein is stable for up to 12 months when stored at -20 to -70°C and should be handled under sterile conditions to avoid contamination .

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