Introduction
Macrophage-derived chemokine (MDC), also known as CCL22, is a small cytokine classified under the CC chemokine family. Similar to other Cys-Cys (CC) cytokine genes, the gene encoding CCL22 is located on the long arm (q arm) of chromosome 16.
MDC exhibits chemotactic activity, attracting various immune cells. It effectively attracts natural killer (NK) cells, chronically activated T lymphocytes, monocytes, and dendritic cells. While it demonstrates mild chemotactic activity towards primarily activated T lymphocytes, it does not attract neutrophils, eosinophils, or resting T lymphocytes. MDC is believed to play a role in guiding activated T lymphocytes to sites of inflammation and may be involved in other aspects of activated T lymphocyte function. This chemokine interacts with the cell surface chemokine receptor CCR4.
CCL22 is predominantly expressed in macrophages, monocyte-derived dendritic cells, and the thymus. It is also found in lymph nodes, the appendix, activated monocytes, and both resting and activated macrophages. Lower expression levels are observed in the lung and spleen, with minimal expression in the small intestine. Within the lymph
Description
Recombinant Mouse CCL22, produced in E. coli, is a non-glycosylated polypeptide chain comprising 68 amino acids, resulting in a molecular mass of 7.8 kDa.
The purification of Mouse CCL22 is achieved through proprietary chromatographic techniques.
Physical Appearance
The product appears as a filtered white lyophilized (freeze-dried) powder.
Formulation
The CCL22 undergoes filtration (0.2 µm) and lyophilization from a concentrated solution containing 20 mM phosphate buffer and 150 mM NaCl at a pH of 7.4.
Solubility
To reconstitute the lyophilized CCL22, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized CCL22 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated state below -18 °C. Once reconstituted, CCL22 should be stored at 4 °C for 2-7 days. For extended storage, it is advisable to store it below -18 °C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Biological Activity
The biological activity of CCL22 is evaluated based on its ability to induce chemotaxis in human activated lymphocytes. This assessment is conducted within a concentration range of 10-100 ng/ml, corresponding to a Specific Activity of 10,000-100,000 IU/mg.
Purity
The purity of CCL22 is determined to be greater than 97.0% through the following analytical methods:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
C-C motif chemokine 22, Small-inducible cytokine A22, Macrophage-derived chemokine, MDC(1-69), Stimulated T-cell chemotactic protein 1, CC chemokine STCP-1, CCL22, MDC, SCYA22, ABCD-1, DC/B-CK, MGC34554, A-152E5.1, CC chemokine ABCD-1, Activated B and dendritic cell-derived, DCBCK.
Amino Acid Sequence
GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD
ICADPRQVWV KKLLHKLS.
