MCP 1 Mouse, His
Description
MCP-1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Monocyte Chemotactic Protein-1, also known as Chemokine (C-C motif) ligand 2, is a small cytokine belonging to the CC chemokine family. It is commonly referred to as Monocyte Chemotactic Protein-1 due to its role in attracting monocytes to sites of inflammation. This protein is crucial in various physiological and pathological processes, including immune response, inflammation, and tissue repair.
Monocyte Chemotactic Protein-1 is a member of the beta chemokine family and is best known for its chemotactic properties, specifically attracting mononuclear cells such as monocytes and lymphocytes . The mouse recombinant version of this protein is produced in Escherichia coli and is tagged with a histidine sequence for purification purposes .
The protein consists of 148 amino acids, including a 23 amino acid signal peptide and a 125 amino acid mature protein . The mature protein is responsible for its biological activity, which includes binding to its receptor, CCR2, on the surface of target cells. This binding initiates a cascade of intracellular signaling events that result in the directed migration of cells towards the source of the chemokine.
Monocyte Chemotactic Protein-1 plays a pivotal role in the immune system by regulating the migration and infiltration of monocytes/macrophages. It is involved in various inflammatory diseases, including rheumatoid arthritis, atherosclerosis, and multiple sclerosis . Additionally, it has been implicated in cancer progression, where it can influence tumor-associated macrophages and promote tumor growth and metastasis.
In the context of bone biology, Monocyte Chemotactic Protein-1 is expressed by mature osteoclasts and osteoblasts and is regulated by nuclear factor kappa B . It is found at sites of bone degradation and tooth eruption, indicating its role in bone remodeling and development .
The recombinant form of Monocyte Chemotactic Protein-1, tagged with histidine, is widely used in research to study its function and role in various biological processes. The histidine tag allows for easy purification and detection of the protein in experimental settings. This recombinant protein is used in cell culture, enzyme-linked immunosorbent assays, and other biochemical assays to investigate its chemotactic properties and signaling pathways.
