MCP 1 Rat
Description
The MCP-1 is purified by proprietary chromatographic techniques.
Product Specs
Lyophilized MCP-1 is stable at room temperature for 3 weeks but should be stored desiccated below -18°C. Reconstituted CCL2 is stable at 4°C for 2-7 days. For long-term storage, freeze at -18°C.
For long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze-thaw cycles.
Product Science Overview
CCL2 is produced by various cell types, including endothelial cells, fibroblasts, epithelial cells, smooth muscle cells, and myeloid cells . It is a potent chemoattractant, meaning it can attract immune cells to the site of inflammation. This chemotactic activity is essential for the body’s immune response, as it helps to recruit immune cells to areas where they are needed to fight off infections or repair damaged tissues .
The expression of CCL2 is inducible and can be triggered by various inflammatory stimuli such as interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) . These stimuli activate the production of CCL2, which then binds to its receptor, CCR2, on the surface of target cells, guiding them to the site of inflammation .
CCL2 is involved in several pathological conditions, including autoimmune diseases like rheumatoid arthritis and multiple sclerosis, inflammatory diseases such as atherosclerosis, and various types of cancer . Its role in these diseases makes it a potential target for therapeutic interventions. However, current strategies to target CCL2 have not yet met early expectations in clinical settings .
Recombinant CCL2, such as the rat recombinant version, is produced using genetic engineering techniques. This involves inserting the gene encoding CCL2 into a host organism, such as bacteria, to produce the protein in large quantities. Recombinant CCL2 is used in research to study its function and role in various diseases, as well as to develop potential therapeutic agents .
