SERPINB5 Human
Serpin Peptidase Inhibitor, Clade B Member 5 Human Recombinant
Cat. No.
BT24313
Source
Escherichia Coli.
Synonyms
PI5, maspin, SERPINB5, serpin peptidase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SERPINB5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 375 amino acids and having a molecular mass of 42.2 KDa.
The SERPINB5 Human is purified by proprietary chromatographic techniques.
The SERPINB5 Human is purified by proprietary chromatographic techniques.
Product Specs
Introduction
SERPINB5 (Maspin) is a tumor suppressor protein belonging to the serine proteinase inhibitor family. It plays a crucial role in embryonic development due to its critical functions in cell adhesion. While found in normal breast and prostate epithelial cells, Maspin is often downregulated in corresponding carcinomas. This protein hinders the growth, invasion, and metastasis of mammary tumors, as well as the invasive capacity of pancreatic ductal adenocarcinoma cells. As a breast tumor suppressor gene, SERPINB5 serves as a vital marker for disease progression in breast neoplasms. Elevated maspin expression is associated with squamous cell carcinoma in non-small-cell lung cancer. Additionally, maspin expression directly correlates with the aggressiveness of ovarian carcinoma. Notably, Maspin does not exhibit serine protease inhibitory activity because it lacks the typical stressed-to-relaxed conformational transition observed in active serpins.
Description
Recombinant human SERPINB5, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 375 amino acids. It has a molecular weight of 42.2 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS with a pH of 7.0.
Solubility
For reconstitution, dissolve the lyophilized SERPINB5 in sterile 18 MΩ-cm H₂O at a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized SERPINB5 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be stored desiccated at a temperature below -18°C. Once reconstituted, SERPINB5 should be stored at 4°C for no more than 2-7 days. For extended storage after reconstitution, add a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by:
(a) High-performance liquid chromatography (HPLC) analysis.
(b) SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
PI5, maspin, SERPINB5, serpin peptidase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5.
Source
Escherichia Coli.
Amino Acid Sequence
MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP.
Product Science Overview
Gene and Protein Structure
Biological Function
Clinical Significance
Pathways and Interactions
Research and Therapeutic Potential
Given its role in tumor suppression, SERPINB5 is a subject of ongoing research. Scientists are exploring its potential as a therapeutic target for cancer treatment. Understanding the mechanisms by which SERPINB5 inhibits tumor growth could lead to the development of new cancer therapies.
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