LYPLA2 Human

The recombinant human LYPLA2 protein is typically expressed in E. coli and is purified using conventional chromatography techniques . The protein is often tagged with a polyhistidine (His) tag at the N-terminus to facilitate purification . The recombinant protein consists of 231 amino acids and has a calculated molecular mass of approximately 26.2 kDa .

LYPLA2 plays a crucial role in regulating the multifunctional lysophospholipids on biological membranes . It is involved in the acylation/deacylation cycle, which is essential for the subcellular distribution of proteins like GAP-43 and H-Ras . The enzyme’s activity is significant in hydrolyzing fatty acids from S-acylated cysteine residues, thereby influencing the localization and function of these proteins .

Recombinant human LYPLA2 is primarily used for research purposes. It is not approved for clinical diagnosis or therapeutic use . Researchers utilize this enzyme to study its role in cellular processes and its potential implications in various diseases.

The recombinant LYPLA2 protein is typically stored at -20°C to -80°C under sterile conditions to maintain its stability . It is recommended to avoid repeated freeze-thaw cycles to preserve the protein’s integrity .