LYPLA1 Mouse

Recombinant Mouse Lysophospholipase I is typically expressed in Escherichia coli and is purified to a high degree, often exceeding 90% purity . This high level of purity is essential for various experimental applications, including SDS-PAGE and mass spectrometry (MS), ensuring that the enzyme’s activity can be studied without interference from other proteins.

The recombinant enzyme is a full-length protein, consisting of 230 amino acids . The sequence of this enzyme includes a His tag at the N-terminus, which facilitates its purification through affinity chromatography. The predicted molecular weight of this enzyme is approximately 26.8 kDa .

While the recombinant form of Lysophospholipase I is not biologically active, it is still valuable for structural and functional studies. Understanding the enzyme’s structure can provide insights into its mechanism of action and its role in lipid metabolism .

Lysophospholipase I has several applications in research and industry. It is used in studies related to lipid metabolism, signal transduction, and the production of lipid mediators. Additionally, it has potential applications in the development of therapeutic agents targeting lipid-related disorders .

Phospholipases, including Lysophospholipase I, have significant industrial and medical potential. They are used in various industries, such as oil refining, health food manufacturing, dairy, and cosmetics . In the medical field, phospholipases can serve as diagnostic markers for microbial infections and as targets for developing inhibitors to prevent diseases associated with their activity .