LSM3 Human

LSM3 Homolog, U6 Small Nuclear RNA Associated Human Recombinant
Cat. No.
BT20698
Source
E.coli.
Synonyms
LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae), U6 snRNA-associated Sm-like protein LSm3, SMX4, USS2, YLR438C.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LSM3 Human Recombinant produced in E. coli is a single polypeptide chain containing 125 amino acids (1-102) and having a molecular mass of 14.3kDa.
LSM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
LSM3, a member of the snRNP Sm protein family, is found in numerous organisms and identified by sequence homology. These proteins possess an Sm sequence motif with two regions separated by a variable-length linker that folds into a loop. LSM3, like other Sm-like proteins, forms a stable heteromer within tri-snRNP particles essential for pre-mRNA splicing.
Description
Recombinant human LSM3, produced in E. coli, is a single polypeptide chain of 125 amino acids (residues 1-102) with a molecular weight of 14.3 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
LSM3 is supplied in a solution at a concentration of 0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 2 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as assessed by SDS-PAGE.
Synonyms
LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae), U6 snRNA-associated Sm-like protein LSm3, SMX4, USS2, YLR438C.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADDVDQ QQTTNTVEEP LDLIRLSLDE RIYVKMRNDR ELRGRLHAYD QHLNMILGDV EETVTTIEID EETYEEIYKS TKRNIPMLFV RGDGVVLVAP PLRVG

Product Science Overview

Function and Mechanism

LSM3 is involved in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex, which is essential for spliceosome assembly. The spliceosome is a complex molecular machine responsible for removing introns from pre-mRNA. LSM3 is also part of the precatalytic spliceosome (spliceosome B complex) .

The heptameric LSM2-8 complex, which includes LSM3, binds specifically to the 3’-terminal U-tract of U6 snRNA. This binding is crucial for the stability and function of U6 snRNA, which is a key component of the spliceosome .

Biological Pathways

LSM3 is associated with several important biological pathways, including:

  • Processing of Capped Intron-Containing Pre-mRNA: This pathway involves the modification and splicing of pre-mRNA to produce mature mRNA.
  • Deadenylation-dependent mRNA Decay: This pathway is responsible for the degradation of mRNA molecules, which is a critical process for regulating gene expression .
Clinical Significance

Mutations or dysregulation of the LSM3 gene can be associated with various diseases. For example, LSM3 has been linked to conditions such as External Pathological Resorption and Tooth Resorption .

Research and Applications

The recombinant form of LSM3, known as Human Recombinant LSM3, is used in various research applications to study its function and role in RNA processing. This recombinant protein is produced using recombinant DNA technology, which allows for the expression of the LSM3 protein in a controlled laboratory environment .

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