LSM1 Human

LSM1 Homolog, U6 Small Nuclear RNA Associated Human Recombinant
Cat. No.
BT20452
Source
Escherichia Coli.
Synonyms
U6 snRNA-associated Sm-like protein LSm1, Cancer-associated Sm-like, Small nuclear ribonuclear CaSm, LSM1, CASM, YJL124C.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LSM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (1-133 a.a.) and having a molecular mass of 17.3kDa. The LSM1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
U6 snRNA-associated Sm-like protein LSm1 (LSM1) plays a role in the degradation of replication-dependent histone mRNA and specifically binds to the 3' terminal U-tract of U6 snRNA. Sm-like proteins are characterized by the Sm sequence motif, which comprises two regions separated by a variable-length linker that folds into a loop structure. The LSM1 protein facilitates interactions between RNA and proteins, as well as structural modifications, which are essential during the assembly of ribosomal subunits. These Sm-like proteins are thought to form a stable heteromer found in tri-snRNP particles, which are crucial for pre-mRNA splicing. Overexpression of LSM1 is observed in pancreatic cancer and various breast cancer cell lines.
Description
Recombinant human LSM1, fused with a 20 amino acid His tag at its N-terminus, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 153 amino acids (amino acids 1-133), with a molecular weight of 17.3 kDa. The purification of LSM1 is carried out using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution.
Formulation
The LSM1 solution is provided at a concentration of 1 mg/ml and contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 10% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the LSM1 protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
U6 snRNA-associated Sm-like protein LSm1, Cancer-associated Sm-like, Small nuclear ribonuclear CaSm, LSM1, CASM, YJL124C.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEY.

Product Science Overview

Structure and Function

The LSM1 protein is part of a heteromeric complex known as the LSm1-7 complex. This complex binds specifically to the 3’-terminal oligo(U) tract of U6 small nuclear RNA (snRNA) and plays a crucial role in the degradation of histone mRNAs, which are unique among eukaryotic mRNAs as they are not polyadenylated . The LSm1-7 complex is also involved in the general process of mRNA degradation, particularly in the deadenylation-dependent decapping of nuclear-transcribed mRNA .

Biological Processes

LSM1 is implicated in several biological processes, including:

  • mRNA Decay: The LSm1-7 complex is essential for the degradation of mRNA, particularly through the deadenylation-dependent decapping pathway .
  • RNA Splicing: By binding to U6 snRNA, LSM1 plays a role in the formation of the U4/U6 snRNP, which is a critical component of the spliceosome .
  • Stem Cell Maintenance: LSM1 is involved in maintaining stem cell populations by regulating mRNA stability and degradation .
Clinical Significance

Increased expression of LSM1 has been associated with the progression of several malignancies, including lung cancer, mesothelioma, and breast cancer . This suggests that LSM1 may play a role in cellular transformation and tumor progression. Additionally, diseases such as Myoclonic-Atonic Epilepsy and Spinal Muscular Atrophy have been linked to mutations or dysregulation of the LSM1 gene .

Research and Applications

Human recombinant LSM1 is used in various research applications to study its role in mRNA degradation and RNA splicing. Understanding the function and regulation of LSM1 can provide insights into the mechanisms of gene expression regulation and the development of certain cancers .

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