Introduction
U6 snRNA-associated Sm-like protein LSm1 (LSM1) plays a role in the degradation of replication-dependent histone mRNA and specifically binds to the 3' terminal U-tract of U6 snRNA. Sm-like proteins are characterized by the Sm sequence motif, which comprises two regions separated by a variable-length linker that folds into a loop structure. The LSM1 protein facilitates interactions between RNA and proteins, as well as structural modifications, which are essential during the assembly of ribosomal subunits. These Sm-like proteins are thought to form a stable heteromer found in tri-snRNP particles, which are crucial for pre-mRNA splicing. Overexpression of LSM1 is observed in pancreatic cancer and various breast cancer cell lines.
Description
Recombinant human LSM1, fused with a 20 amino acid His tag at its N-terminus, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 153 amino acids (amino acids 1-133), with a molecular weight of 17.3 kDa. The purification of LSM1 is carried out using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution.
Formulation
The LSM1 solution is provided at a concentration of 1 mg/ml and contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 10% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the LSM1 protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
U6 snRNA-associated Sm-like protein LSm1, Cancer-associated Sm-like, Small nuclear ribonuclear CaSm, LSM1, CASM, YJL124C.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEY.