LSM2 Human

LSM2 Homolog, U6 Small Nuclear RNA Associated Human Recombinant
Cat. No.
BT20649
Source
E.coli.
Synonyms
LSM2 homolog U6 small nuclear RNA associated (S. cerevisiae), Small nuclear ribonuclear protein D homolog, chromosome 6 open reading frame 28, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W, Protein G7b, snRNP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LSM2 Human Recombinant produced in E. coli is a single polypeptide chain containing 119 amino acids (1-95) and having a molecular mass of 13.4kDa.
LSM2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
LSM2, a member of the snRNP Sm protein family, belongs to a group of proteins identified in various organisms based on their sequence homology to the Sm protein family. Characterized by the Sm sequence motif, these proteins feature two regions separated by a variable-length linker that folds into a loop. Sm-like proteins are thought to form a stable heteromer found in tri-snRNP particles, which are crucial for pre-mRNA splicing. LSM2 specifically binds to the 3'-terminal U-tract of U6 snRNA, playing a vital role in pre-mRNA splicing.
Description
Recombinant human LSM2, produced in E. coli, is a single polypeptide chain comprising 119 amino acids (1-95) with a molecular weight of 13.4 kDa. The protein is fused to a 24-amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The LSM2 solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 5 mM DTT, and 40% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of LSM2 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
LSM2 homolog U6 small nuclear RNA associated (S. cerevisiae), Small nuclear ribonuclear protein D homolog, chromosome 6 open reading frame 28, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W, Protein G7b, snRNP.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLFYSF FKSLVGKDVV VELKNDLSIC GTLHSVDQYL NIKLTDISVT DPEKYPHMLS VKNCFIRGSV VRYVQLPADE VDTQLLQDAA RKEALQQKQ

Product Science Overview

Gene and Protein Structure

The LSM2 gene is located on chromosome 6 in humans and is also known by several aliases, including C6orf28, G7B, and YBL026W . The protein encoded by LSM2 contains the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop . This structure is crucial for its function in RNA binding and processing.

Function and Mechanism

LSM2 is a component of the U4/U6-U5 tri-snRNP complex, which is involved in spliceosome assembly . The spliceosome is a complex molecular machine responsible for removing introns from pre-mRNA, a critical step in mRNA maturation. LSM2, along with other LSm proteins, forms a stable heteromer that binds specifically to the 3’-terminal oligo(U) tract of U6 snRNA . This binding is essential for the formation of the U4/U6 duplex, which is a prerequisite for the assembly of the spliceosome .

Additionally, LSM2 is involved in mRNA degradation pathways, including deadenylation-dependent mRNA decay . This process is vital for regulating mRNA stability and, consequently, gene expression.

Biological Significance

The proper functioning of LSM2 is crucial for cellular RNA metabolism. Dysregulation of LSM2 and its associated pathways can lead to various diseases. For instance, LSM2 has been implicated in mixed connective tissue disease and cat-scratch disease . Moreover, its role in RNA splicing and degradation makes it a potential target for therapeutic interventions in diseases where RNA processing is disrupted.

Research and Applications

Human recombinant LSM2 is used in various research applications to study its function and interactions. By expressing and purifying recombinant LSM2, researchers can investigate its role in RNA metabolism and its potential as a therapeutic target. Studies have shown that LSM2 interacts with other proteins such as DDX20, LSM3, LSM7, and LSM8, highlighting its involvement in complex RNA processing networks .

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