LMNA Human
Prelamin-A/C, Lamin-A/C, 70 kDa lamin, LMNA, LMN1, Renal carcinoma antigen NY-REN-32, Progerin.
Greater than 90.0% as determined by SDS-PAGE.
Description
LMNA Human Recombinant fused with a His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 614 amino acids and having a molecular mass of 68.0kDa. The LMNA is purified by proprietary chromatographic techniques.
Product Specs
Prelamin-A/C, Lamin-A/C, 70 kDa lamin, LMNA, LMN1, Renal carcinoma antigen NY-REN-32, Progerin.
MAHHHHHHVG TGSNDDDDKS PDMETPSQRR ATRSGAQASS TPLSPTRITR LQEKEDLQEL NDRLAVYIDR VRSLETENAG LRLRITESEE VVSREVSGI KAAYEAELGD ARKTLDSVAK ERARLQLELS KVREEFKELK ARNTKKEGDL IAAQARLKDL EALLNSKEAA LSTALSEKRT LEGELHDLRG QVAKLEAALG EAKKQLQDEM LRRVDAENRL QTMKEELDFQ KNIYSEELRE TKRRHETRLV EIDNGKQREF ESRLADALQE LRAQHEDQVE QYKKELEKTY SAKLDNARQS AERNSNLVGA AHEELQQSRI RIDSLSAQLS QLQKQLAAKE AKLRDLEDSL ARERDTSRRL LAEKEREMAE MRARMQQQLD EYQELLDIKL ALDMEIHAYR KLLEGEEERL RLSPSPTSQR SRGRASSHSS QTQGGGSVTK KRKLESTESR SSFSQHARTS GRVAVEEVDE EGKFVRLRNK SNEDQSMGNW QIKRQNGDDP LLTYRFPPKF TLKAGQVVTI WAAGAGATHS PPTDLVWKAQ NTWGCGNSLR TALINSTGEE VAMRKLVRSV TVVEDDEDED GDDLLHHHHG SHCSSSGDPA EYNLRSRTVL CGTCGQPADK ASASGSGAQS PQNCSIM
Product Science Overview
Lamin A/C is a crucial protein found in the nuclear lamina, a dense fibrillar network inside the nucleus of eukaryotic cells. This network provides structural support and regulates various nuclear activities, including DNA replication, transcription, and chromatin organization. Lamin A/C is encoded by the LMNA gene and exists in two main isoforms: lamin A and lamin C, which are produced through alternative splicing.
Lamin A/C belongs to the type V intermediate filament proteins. These proteins share a conserved tripartite structure consisting of a central α-helical coiled-coil domain flanked by a non-helical N-terminal head and a C-terminal tail domain . The tail domain contains an immunoglobulin (Ig)-fold, which is crucial for the protein’s function .
The primary role of lamin A/C is to provide mechanical support to the nucleus. It also plays a significant role in various nuclear processes, including:
- DNA replication and repair: Lamin A/C interacts with DNA and other nuclear proteins to facilitate these processes.
- Gene expression regulation: Lamin A/C is involved in the organization of chromatin, thereby influencing gene expression.
- Nuclear signaling: Lamin A/C participates in various signaling pathways that regulate cell growth, differentiation, and apoptosis.
Mutations in the LMNA gene can lead to a group of diseases known as laminopathies. These diseases include muscular dystrophies, lipodystrophies, neuropathies, and premature aging syndromes . The molecular mechanisms by which these mutations cause disease are still not fully understood, but they are believed to disrupt the structural integrity of the nuclear lamina and impair its functions.
Recombinant lamin A/C is produced using recombinant DNA technology, which involves inserting the LMNA gene into an expression vector and introducing it into a host cell, typically E. coli or yeast. The host cells then produce the lamin A/C protein, which can be purified for research or therapeutic use.
Recombinant lamin A/C is widely used in research to study the structure and function of the nuclear lamina, as well as the molecular mechanisms underlying laminopathies. It is also used in drug discovery and development, particularly in screening for compounds that can modulate lamin A/C function or expression.
