Lamin-A Human

Lamin-A is a crucial protein that plays a significant role in maintaining the structural integrity of the cell nucleus. It is a type of intermediate filament protein that forms a dense network called the nuclear lamina, located just beneath the inner nuclear membrane. Lamin-A, along with Lamin-C, is encoded by the LMNA gene. These proteins are essential for various cellular functions, including DNA replication, chromatin organization, and cell cycle regulation.

Lamin-A is synthesized as a precursor protein called prelamin-A, which undergoes several post-translational modifications to become mature Lamin-A. These modifications include farnesylation, proteolytic cleavage, and methylation. The mature Lamin-A protein is approximately 74 kDa in size and is known for its role in providing mechanical support to the nucleus and regulating gene expression.

Human recombinant Lamin-A is produced using recombinant DNA technology, which involves inserting the human LMNA gene into an expression vector and introducing it into a host cell, such as E. coli or yeast. The host cells then produce Lamin-A, which can be purified and used for various research and therapeutic purposes.

Recombinant Lamin-A is widely used in scientific research to study the functions and interactions of nuclear lamins. It is also used in the development of therapeutic strategies for diseases associated with LMNA mutations, such as Hutchinson-Gilford Progeria Syndrome (HGPS) and various forms of muscular dystrophy.