LGALS8 Mouse

Galectin-8 Mouse Recombinant
Cat. No.
BT11552
Source
Escherichia Coli.
Synonyms
Galectin-8, Gal-8, LGALS-8, AI326142, D13Ertd524e, 1200015E08Rik.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS8 mouse Recombinant produced E. coli is a single polypeptide chain containing 339 amino acids (1-316) and having a molecular mass of 38kDa.
LGALS8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
LGALS8, a prostate-specific antigen, exhibits exclusive overexpression in malignant tumors, making it a valuable supplementary marker for identifying malignancies. This protein belongs to the galectin family, which plays a crucial role in mediating cell-cell and cell-matrix interactions, similar to the selectin family of C-type lectins.
Description
Recombinant LGALS8, produced in E. coli, is a single polypeptide chain consisting of 339 amino acids (residues 1-316) and possessing a molecular weight of 38kDa. This protein is engineered with a 23 amino acid His-tag fused at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile-filtered solution, clear and colorless.
Formulation
The LGALS8 solution is supplied at a concentration of 0.5mg/ml and is formulated in 20mM Tris-HCl buffer at pH 8.0, containing 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be minimized.
Purity
The purity of the protein is determined to be greater than 90.0% using SDS-PAGE analysis.
Assay Conditions
1. Prepare a mixture of equal volumes of human blood and Alsever's solution (pH 7.0). Alsever's solution composition: NaCl (0.42g), Sodium citrate (0.8g), Citric acid (0.055g), and D-glucose (2.05g) dissolved in 100 ml of distilled water. 2. Centrifuge the blood-Alsever's solution mixture at 15,000 rpm for 10 minutes. Wash the resulting pellet four times with PBS. 3. Resuspend the packed cells in a 0.5mg/ml trypsin-EDTA solution to achieve a 4% red cell suspension. 4. Incubate the cell suspension for 1 hour at 37°C. Wash the cells four times with PBS. 5. Resuspend the packed cells in PBS to obtain a 4% red cell suspension. 6. Dispense 50µl of 0.5% BSA in 0.15M NaCl solution and 25µl of the 4% red cell suspension in PBS into separate wells of a U-shaped well plate. 7. Add 25µl of serially diluted galectin protein in PBS to each well of the plate (using a round-bottom 96-well plate). 8. Incubate the plate for 30 minutes at room temperature and visually assess for agglutination.
Biological Activity
The ED₅₀ (effective dose for 50% response) is approximately 2µg/ml, as determined by the protein's ability to agglutinate human red blood cells.
Synonyms
Galectin-8, Gal-8, LGALS-8, AI326142, D13Ertd524e, 1200015E08Rik.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLSLNNL QNIIYNPIIP YVGTITEQLK PGSLIVIRGH VPKDSERFQV DFQLGNSLKP RADVAFHFNP RFKRSSCIVC NTLTQEKWGW EEITYDMPFR KEKSFEIVFM VLKNKFQVAV NGRHVLLYAH RISPEQIDTV GIYGKVNIHS IGFRFSSDLQ
SMETSALGLT QINRENIQKP GKLQLSLPFE ARLNASMGPG RTVVIKGEVN TNARSFNVDL VAGKTRDIAL HLNPRLNVKA FVRNSFLQDA WGEEERNITC FPFSSGMYFE MIIYCDVREF KVAINGVHSL EYKHRFKDLS SIDTLSVDGD IRLLDVRSW.

Product Science Overview

Introduction

Galectin-8, also known as Gal-8, is a member of the galectin family, which are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains (CRDs). Galectin-8 is a tandem-repeat type galectin, containing two CRDs connected by a linker peptide . This protein is expressed in various tissues and has been implicated in numerous biological processes, including cell adhesion, migration, and angiogenesis .

Structure and Expression

Galectin-8 was initially cloned from a rat liver cDNA library and is composed of 316 amino acids, with a molecular weight of approximately 35 kDa . The N- and C-terminal domains of Galectin-8 share 35% homology and contain sequence motifs conserved among most CRDs of galectins . The mouse recombinant version of Galectin-8 is expressed in Escherichia coli and has a purity of over 90%, making it suitable for various applications such as SDS-PAGE and mass spectrometry .

Biological Functions

Galectin-8 plays a crucial role in various biological processes. It is involved in the regulation of vascular and lymphatic angiogenesis, which is the formation of new blood and lymphatic vessels . This protein has been shown to control capillary-tube formation, endothelial cell (EC) migration, and in vivo angiogenesis . Additionally, Galectin-8 interacts with specific ligands such as podoplanin in lymphatic vessels and CD166 (ALCAM) in vascular ECs .

Role in Disease and Therapeutic Potential

Galectin-8 has significant implications in tumor biology. It is expressed in both normal and tumor-associated blood vessels, as well as in lymphatic endothelial cells . The protein’s role in tumor angiogenesis and metastasis is of particular interest, as modulating Galectin-8-glycan interactions could potentially block lymphatic-vascular connections vital for metastasis . Furthermore, Galectin-8 has been shown to restrict the proliferation of infecting pathogens by targeting them for autophagy, making it a potential therapeutic target for infectious diseases .

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